The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae

PeerJ
Nicholas C ThomasP C Ronald

Abstract

Rice (Oryza sativa) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. oryzae (Xoo) infection. We previously demonstrated that expressing a chimeric protein containing the ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and the XA21 endodomain (EFR:XA21) in rice does not confer robust resistance to Xoo. To test if the XA21 ectodomain is required for Xoo resistance, we produced transgenic rice lines expressing a chimeric protein consisting of the XA21 ectodomain and EFR endodomain (XA21:EFR) and inoculated these lines with Xoo. We also tested if the XA21:EFR rice plants respond to a synthetic sulfated 21 amino acid derivative (RaxX21-sY) of the activator of XA21-mediated immunity, RaxX. We found that five independently transformed XA21:EFR rice lines displayed resistance to Xoo as measured by lesion length analysis, and showed that five lines share characteristic markers of the XA21 defense response (generation of reactive oxygen species and defense response gene expression) after treatment with RaxX21-sY. Our results indicate that expression of the XA21:EFR chimeric receptor in rice confers resistance to Xoo. These results suggest that the endodomain of the EFR and XA21 immune recepto...Continue Reading

References

Mar 15, 1997·The Biochemical Journal·P Wojtaszek
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 17, 2005·Plant & Cell Physiology·Annabelle Decreux, Johan Messiaen
Jan 21, 2006·PLoS Pathogens·Christopher Dardick, Pamela Ronald
Jul 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hanae KakuNaoto Shibuya
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Nov 7, 2008·Molecular Plant-microbe Interactions : MPMI·Ryota TakaiFang-Sik Che
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alexandre BrutusGiulia De Lorenzo
Nov 13, 2010·The Plant Journal : for Cell and Molecular Biology·Kyutaro KishimotoYoko Nishizawa
Nov 26, 2010·Science·Pamela C Ronald, Bruce Beutler
Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Wenqi LiFengquan Liu
Apr 29, 2014·Molecular Cell·Alberto P Macho, Cyril Zipfel
Nov 28, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Benjamin SchwessingerPamela C Ronald
Jan 15, 2016·Molecular Plant Pathology·Tong WeiPamela C Ronald

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
genotyping

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.