The RING domain and the L79 residue of Z protein are involved in both the rescue of nucleocapsids and the incorporation of glycoproteins into infectious chimeric arenavirus-like particles.

Journal of Virology
Juan Cruz CasabonaNora López

Abstract

Arenaviruses, such as Tacaribe virus (TacV) and its closely related pathogenic Junin virus (JunV), are enveloped viruses with a bipartite negative-sense RNA genome that encodes the nucleocapsid protein (N), the precursor of the envelope glycoprotein complex (GP), the polymerase (L), and a RING finger protein (Z), which is the driving force of arenavirus budding. We have established a plasmid-based system which allowed the successful packaging of TacV-like nucleocapsids along with Z and GP of JunV into infectious virus-like particles (VLPs). By coexpressing different combinations of the system components, followed by biochemical analysis of the VLPs, the requirements for the assembly of both N and GP into particles were defined. We found that coexpression of N with Z protein in the absence of minigenome and other viral proteins was sufficient to recruit N within lipid-enveloped Z-containing VLPs. In addition, whereas GP was not required for the incorporation of N, coexpression of N substantially enhanced the ratio of GP to Z into VLPs. Disruption of the RING structure or mutation of residue L79 to alanine within Z protein, although it had no effect on Z self-budding, severely impaired VLP infectivity. These mutations drastically...Continue Reading

References

Nov 1, 1990·Nature·B MossT R Fuerst
Jul 1, 1985·The Journal of General Virology·C R HowardM J Buchmeier
Jan 1, 1987·Current Topics in Microbiology and Immunology·D H Bishop, D D Auperin
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
May 1, 1996·Virology·M D BowenS T Nichol
Jul 1, 1996·Research in Virology·C RossiM T Franze-Fernández
Apr 18, 2000·The Journal of General Virology·N LópezM T Franze-Fernández
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alex KentsisKatherine L B Borden
Sep 13, 2003·Journal of Virology·Rodrigo JácamoMaría T Franze-Fernández
Oct 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mar PerezJuan C de la Torre
Mar 3, 2004·Journal of Virology·Tatjana I CornuJuan Carlos de la Torre
Aug 10, 2004·Current Topics in Microbiology and Immunology·A P Schmitt, R A Lamb
Sep 29, 2004·Journal of Virology·Mar PerezJuan Carlos de la Torre
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Eiji Morita, Wesley I Sundquist
Mar 31, 2006·Journal of Virology·Shuzo UrataJiro Yasuda
Oct 3, 2006·PLoS Pathogens·Takeshi NodaYoshihiro Kawaoka
Nov 7, 2006·Virology Journal·Thomas StreckerWolfgang Garten
Dec 7, 2007·Antiviral Research·Delia A EnriaZaida Sánchez
Jul 8, 2008·Current Opinion in Microbiology·Rémi N CharrelSébastien Emonet

❮ Previous
Next ❯

Citations

Nov 6, 2012·BMC Biotechnology·Cristina S BorioMario E Lozano
Jan 15, 2010·Journal of Virology·Katrin SchlieWolfgang Garten
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
Jul 24, 2010·Journal of Biomedicine & Biotechnology·S E GoñiM E Lozano
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Oct 1, 2011·Journal of Virology·Maria Eugenia LoureiroNora Lopez
Dec 4, 2012·Viruses·Shuzo Urata, Jiro Yasuda
Dec 4, 2012·Viruses·Dominique J BurriAntonella Pasquato
Dec 4, 2012·Viruses·Sarah Katharina FehlingThomas Strecker
Jan 25, 2013·Viruses·Juan C Zapata, Maria S Salvato
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Jan 18, 2011·Microbes and Infection·Ricardo M GómezMirta Schattner
Mar 19, 2013·Zoonoses and Public Health·R KoukuntlaR Flick
Jul 26, 2013·The Journal of General Virology·Yinjie ZhangCheng Yang
Feb 28, 2015·The Journal of General Virology·Shuzo Urata, Jiro Yasuda
Jul 6, 2010·Antiviral Research·Claudia S SepúlvedaElsa B Damonte
Jul 13, 2018·PLoS Pathogens·María Eugenia LoureiroElina Zúñiga
Jan 21, 2011·Journal of Virology·Olena ShtankoYoshihiro Kawaoka
Dec 18, 2013·Journal of Virology·Juan C ZapataMaria S Salvato
Mar 29, 2019·Frontiers in Immunology·Morgan E Brisse, Hinh Ly
Feb 1, 2019·Pathogens·María Eugenia LoureiroNora López
Aug 26, 2021·Journal of Virology·Jesse D Pyle, Sean P J Whelan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.