The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability

Plant Physiology
Laurens PauwelsAlain Goossens

Abstract

Jasmonate (JA) signaling in plants is mediated by the JASMONATE ZIM-DOMAIN (JAZ) proteins that repress the activity of several transcription factors regulating JA-inducible gene expression. The hormone JA-isoleucine triggers the interaction of JAZ repressor proteins with the F-box protein CORONATINE INSENSITIVE1 (COI1), part of an S-phase kinase-associated protein1/Cullin1/F-box protein COI1 (SCF(COI1)) E3 ubiquitin ligase complex, and their degradation by the 26S proteasome. In Arabidopsis (Arabidopsis thaliana), the JAZ family consists of 13 members. The level of redundancy or specificity among these members is currently not well understood. Here, we characterized JAZ12, encoded by a highly expressed JAZ gene. JAZ12 interacted with the transcription factors MYC2, MYC3, and MYC4 in vivo and repressed MYC2 activity. Using tandem affinity purification, we found JAZ12 to interact with SCF(COI1) components, matching with observed in vivo ubiquitination and with rapid degradation after treatment with JA. In contrast to the other JAZ proteins, JAZ12 also interacted directly with the E3 RING ligase KEEP ON GOING (KEG), a known repressor of the ABSCISIC ACID INSENSITIVE5 transcription factor in abscisic acid signaling. To study the fu...Continue Reading

Associated Datasets

Aug 7, 2017·Alain GoossensAndrĂŠs Ritter
Aug 7, 2017·Plant Systems Biology data submissionAlain Goossens

Citations

May 3, 2016·Plants·Astrid Nagels DurandAlain Goossens
Apr 18, 2016·Plant Molecular Biology·Jonas GoossensAlain Goossens
Aug 9, 2016·Plant & Cell Physiology·Astrid Nagels DurandAlain Goossens
Sep 20, 2016·BMC Biology·Antoine Larrieu, Teva Vernoux
Dec 8, 2016·Molecular Plant Pathology·Bangjun Zhou, Lirong Zeng
Mar 15, 2018·Annual Review of Plant Biology·Gregg A HoweAbraham J Koo
May 5, 2018·Journal of Experimental Botany·Alexandra MiricescuEmmanuelle Graciet
Mar 9, 2018·Molecular & Cellular Proteomics : MCP·Dior R Kelley
Aug 30, 2018·Plant, Cell & Environment·Sylwia StrukSofie Goormachtig
Sep 3, 2020·Journal of Integrative Plant Biology·Chenyang GaoDingzhong Tang
Apr 9, 2020·The New Phytologist·Patricia Fernández-CalvoAndrés Ritter
Feb 23, 2021·The Plant Journal : for Cell and Molecular Biology·Niels AertsSaskia C M Van Wees
Mar 7, 2021·Trends in Plant Science·Evi CeulemansAlain Goossens
Apr 4, 2021·International Journal of Molecular Sciences·Hai Liu, Michael P Timko
Jun 1, 2021·Trends in Plant Science·Vishal Varshney, Manoj Majee

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