The RING finger protein SNURF is a bifunctional protein possessing DNA binding activity

The Journal of Biological Chemistry
Marika HäkliJorma J Palvimo

Abstract

The small nuclear C(3)HC(4) finger protein (SNURF), RNF4, acts as transcriptional coactivator for both steroid-dependent and -independent promoters such as those driven by androgen response elements and GC boxes, respectively. However, SNURF does not possess intrinsic transcription activation function, and the precise molecular mechanism of its action is unknown. We have studied herein the interaction of SNURF with DNA in vitro. SNURF binds to linear double-stranded DNA with no apparent sequence specificity in a cooperative fashion that is highly dependent on the length of the DNA fragment used. SNURF interacts efficiently with both supercoiled circular and four-way junction DNA, and importantly, it also recognizes nucleosomes. An intact RING structure of SNURF is not mandatory for DNA binding, whereas mutations of specific positively charged residues in the N terminus (amino acids 8-11) abolish DNA binding. Interestingly, the ability of SNURF to interact with DNA is associated with its capability to enhance transcription from promoters containing GC box elements. Because SNURF can interact with both DNA and protein (transcription) factors, it may promote assembly of nucleoprotein structures.

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Citations

Apr 5, 2014·Nature Structural & Molecular Biology·Helle D Ulrich
Mar 2, 2016·Biomolecules·Minghua Nie, Michael N Boddy
Feb 28, 2002·Biochemical and Biophysical Research Communications·Sirpa J HirvonenJorma J Palvimo
Mar 11, 2003·Technology in Cancer Research & Treatment·Roberta Piva, Roberto Gambari
Jul 23, 2003·Molecular Reproduction and Development·Raffaela PeroLorenzo Chiariotti
May 16, 2007·The Journal of Biological Chemistry·Ana KosoyMatthew J O'Connell
Aug 30, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alexander J Garvin, Joanna R Morris
Oct 1, 2019·Proteomics·Ramesh Kumar, Kanaga Sabapathy
Nov 24, 2001·The Journal of Biological Chemistry·Raffaela PeroLorenzo Chiariotti
Jan 31, 2019·Frontiers in Neuroscience·Cristine Alves da CostaFrédéric Checler
Jun 3, 2021·International Journal of Molecular Sciences·Ya-Chu ChangAnja-Katrin Bielinsky

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