The ring-infected erythrocyte surface antigen protein of Plasmodium falciparum is phosphorylated upon association with the host cell membrane

Molecular and Biochemical Parasitology
M FoleyR F Anders

Abstract

The ring-infected erythrocyte surface antigen (RESA) is a 155-kDa malarial polypeptide which is released from merozoites and becomes associated with the erythrocyte membrane at the time of invasion. Inside-out vesicles (IOVs) prepared from Plasmodium falciparum-infected erythrocytes contain RESA, presumably bound to the membrane skeleton, as it is largely insoluble in Triton X-100. When these IOVs were incubated with [gamma-32P]ATP, a 155-kDa polypeptide was labeled in IOVs from infected, but not from uninfected erythrocytes. Immunoprecipitation using specific rabbit antisera confirmed that RESA is indeed a phosphoprotein. Phosphoamino acid analysis revealed phosphoserine and a small amount of phosphothreonine, but no phosphotyrosine. Labeling of intact parasitized erythrocytes with inorganic [32P]phosphate for several hours in culture resulted in RESA in Triton-insoluble extracts being phosphorylated. Labeling of synchronized parasites showed that RESA was phosphorylated only when it became associated with the erythrocyte membrane, and although RESA was abundant in mature parasites, it was not phosphorylated. RESA, released into the culture supernatants during the growth of P. falciparum, bound to IOVs prepared from normal uni...Continue Reading

References

Aug 20, 1976·Science·W Trager, J B Jensen
Jul 1, 1988·Journal of Cellular Biochemistry·C D CianciJ S Morrow
Dec 1, 1988·Molecular and Biochemical Parasitology·R L CoppelR F Anders
Feb 12, 1986·Journal of Immunological Methods·P E CrewtherR F Anders

❮ Previous
Next ❯

Citations

Dec 17, 2009·Veterinary Research Communications·Ahmed Ibrahem I BadawyCarlos Hermosilla
Nov 1, 1995·Parasitology Today·M Foley, L Tilley
Mar 1, 1997·Molecular and Biochemical Parasitology·J F KunR F Anders
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S SchulmanR S Schwartz
Aug 11, 1991·British Journal of Haematology·H L ShearR L Nagel
Jul 15, 1992·European Journal of Biochemistry·B W SuetterlinR M Franklin
Jul 1, 1995·The Journal of Eukaryotic Microbiology·A BonninP Camerlynck
Mar 29, 2014·Parasitology·E R Pesce, G L Blatch
Dec 21, 2011·IUBMB Life·Melanie Rug, Alexander G Maier
Jun 1, 1993·Baillière's Clinical Haematology·G PasvolB Clough
Jan 19, 2015·Trends in Parasitology·Lars HviidFreya J I Fowkes
Sep 2, 2016·Microbiology and Molecular Biology Reviews : MMBR·Jan D WarnckeHans-Peter Beck
Jul 7, 2017·Science Translational Medicine·Papa Alioune NdourUNKNOWN French Artesunate Working Group
Jun 24, 1998·Biochemical and Biophysical Research Communications·D YokoyamaT Matsumura
Apr 9, 2004·Seminars in Hematology·Brian M CookeRoss L Coppel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.