PMID: 9644218Jun 30, 1998Paper

The rise of nuclear and cytosolic Ca2+ can be uncoupled in HeLa cells

Pflügers Archiv : European journal of physiology
P P LuiC Y Lee

Abstract

It has long been assumed that ionized Ca2+ is transmitted from the cytosol to the cell nucleus through the nuclear pore complexes to trigger many nuclear functions such as gene transcription after agonist stimulation. However, this hypothesis has been challenged recently. In the present study, we have investigated the interplay of Ca2+ in the cytosol and nucleus of HeLa cells upon histamine stimulation by using confocal microscopy. In resting cells, addition of histamine (50 microM) produced synchronous Ca2+ signals in the cytosol and nucleus with a stronger fluo-3 emission in the nucleus. Our results also demonstrate that the rise of cytosolic and nuclear free Ca2+ can be uncoupled after histamine stimulation. This assertion is supported by the following observations: (1) when heparin was injected into the cytosol, the increase in fluo-3 fluorescence in the cytosol mediated by histamine was almost eliminated while that in the nucleus still occurred; (2) when heparin was in the nucleus, the increase in the free nucleoplasmic Ca2+ concentration ([Ca2+]n) elicited by histamine was abolished while the rise of cytosolic Ca2+ level ([Ca2+]c) was seen; (3) when we injected inositol 1,4,5-trisphosphate (IP3) directly into the nucleus,...Continue Reading

Citations

Mar 11, 2006·The Cerebellum·Sergey M Marchenko, Roger C Thomas
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·M F LeiteM H Nathanson
Feb 24, 2009·Canadian Journal of Physiology and Pharmacology·Ghassan BkailyDanielle Jacques
Oct 5, 2011·Cellular and Molecular Life Sciences : CMLS·Enrico Zampese, Paola Pizzo
Dec 15, 2010·Cell Calcium·Maria Teresa Alonso, Javier García-Sancho
Jul 6, 2014·Journal of Molecular and Cellular Cardiology·Cristián IbarraSergio Lavandero
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Rebecca K Y LeeS K Kong
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Oct 28, 2006·Cell Calcium·María Teresa AlonsoJavier García-Sancho
Jun 23, 2007·Cell Calcium·Pablo ChameroJavier García-Sancho

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