The RNA-Binding Chaperone Hfq Is an Important Global Regulator of Gene Expression in Pasteurella multocida and Plays a Crucial Role in Production of a Number of Virulence Factors, Including Hyaluronic Acid Capsule

Infection and Immunity
Marianne MégrozJ D Boyce

Abstract

The Gram-negative bacterium Pasteurella multocida is the causative agent of a number of economically important animal diseases, including avian fowl cholera. Numerous P. multocida virulence factors have been identified, including capsule, lipopolysaccharide (LPS), and filamentous hemagglutinin, but little is known about how the expression of these virulence factors is regulated. Hfq is an RNA-binding protein that facilitates riboregulation via interaction with small noncoding RNA (sRNA) molecules and their mRNA targets. Here, we show that a P. multocida hfq mutant produces significantly less hyaluronic acid capsule during all growth phases and displays reduced in vivo fitness. Transcriptional and proteomic analyses of the hfq mutant during mid-exponential-phase growth revealed altered transcript levels for 128 genes and altered protein levels for 78 proteins. Further proteomic analyses of the hfq mutant during the early exponential growth phase identified 106 proteins that were produced at altered levels. Both the transcript and protein levels for genes/proteins involved in capsule biosynthesis were reduced in the hfq mutant, as were the levels of the filamentous hemagglutinin protein PfhB2 and its secretion partner LspB2. In c...Continue Reading

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Citations

Aug 22, 2017·Toxins·Marina Harper, John Dallas Boyce
May 12, 2017·Clinical Microbiology Reviews·Udoka OkaroBurt Anderson
Mar 9, 2018·Frontiers in Microbiology·Karin L MeibomRizlan Bernier-Latmani
Oct 24, 2020·Pathogens and Disease·Josicelli Souza CrispimDenise Mara Soares Bazzolli
Nov 5, 2019·Research in Veterinary Science·Lijun GuanZhanqin Zhao
Feb 6, 2021·Life·Macauley J GreenPhilip M Williams
Aug 14, 2020·International Journal of Biological Macromolecules·Vijay GunasekaranPonnusami V

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