The RNA Binding Protein Igf2bp1 Is Required for Zebrafish RGC Axon Outgrowth In Vivo

PloS One
John A GaynesChi-Bin Chien

Abstract

Attractive growth cone turning requires Igf2bp1-dependent local translation of β-actin mRNA in response to external cues in vitro. While in vivo studies have shown that Igf2bp1 is required for cell migration and axon terminal branching, a requirement for Igf2bp1 function during axon outgrowth has not been demonstrated. Using a timelapse assay in the zebrafish retinotectal system, we demonstrate that the β-actin 3'UTR is sufficient to target local translation of the photoconvertible fluorescent protein Kaede in growth cones of pathfinding retinal ganglion cells (RGCs) in vivo. Igf2bp1 knockdown reduced RGC axonal outgrowth and tectal coverage and retinal cell survival. RGC-specific expression of a phosphomimetic Igf2bp1 reduced the density of axonal projections in the optic tract while sparing RGCs, demonstrating for the first time that Igf2bp1 is required during axon outgrowth in vivo. Therefore, regulation of local translation mediated by Igf2bp proteins may be required at all stages of axon development.

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Citations

May 3, 2016·Wiley Interdisciplinary Reviews. RNA·Soma DashSalil A Lachke
Dec 4, 2020·Journal of Neuroscience Research·Lingyan XingGang Chen
Jul 14, 2021·Animal Biotechnology·Hongfei LiuChuanying Pan

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Datasets Mentioned

BETA
EB781185

Methods Mentioned

BETA
transgenic
PCR
restriction digest
dissecting

Software Mentioned

FluoRender
ASW
Fiji
ImageJ
ClustalW
SPOT
BLAST
FV10
Imaris

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