The RNA binding protein RBMS3 inhibits the metastasis of breast cancer by regulating Twist1 expression

Journal of Experimental & Clinical Cancer Research : CR
Lei ZhuJi-Fu Wei

Abstract

Metastasis remains the biggest obstacle for breast cancer treatment. Therefore, identification of specific biomarker of metastasis is very necessary. The RNA binding protein 3 (RBMS3) acts as a tumor suppressor in various cancers. Whereas, its role and underlying molecular mechanism in breast cancer is far from elucidated. Quantitative real-time PCR and western blots were carried out to determine the expression of RBMS3 in breast cancer cells and tissues. Transwell and in vivo metastasis assay were conducted to investigate the effects of RBMS3 on migration, invasion and metastasis of breast cancer cells. Transcriptome sequencing was applied to screen out the differential gene expression affected by RBMS3. RNA immunoprecipitation assay combined with luciferase reporter assay were performed to explore the direct correlation between RBMS3 and Twist1 mRNA. RBMS3 was downregulated in breast cancer and ectopic expression of RBMS3 contributed to inhibition of cell migration, invasion in vitro and lung metastasis in vivo. Furthermore, RBMS3 negatively regulated Twsit1 expression via directly binding to 3'-UTR of Twist1 mRNA, and thereby decreased Twist1-induced expression of matrix metalloproteinase 2 (MMP2). Additionally, Twist1-induc...Continue Reading

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Citations

Jan 29, 2020·Journal of Experimental & Clinical Cancer Research : CR·Lei ZhuJi-Fu Wei
Aug 15, 2020·Frontiers in Pharmacology·Qianxuan LuoMeichun Deng
Dec 11, 2019·The Journal of Thoracic and Cardiovascular Surgery·Ari A Mennander
Mar 16, 2021·Frontiers in Cell and Developmental Biology·John GreeneStephen P Finn
Apr 30, 2021·Technology in Cancer Research & Treatment·Chunyang WangLiang Yu
Nov 23, 2021·Frontiers in Oncology·Zhigang LiJing Tang

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Methods Mentioned

BETA
PCR
Assay
immunoprecipitation
transfection
immunoprecipitation assay
RIP

Software Mentioned

Cutadapt
Gene Set Enrichment Analysis ( GSEA )
R
Cufflinks
TopHat2
Kmplotter
Graphpad Prism

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