The RNA-binding site of bacteriophage Qbeta coat protein.

The Journal of Biological Chemistry
F LimD S Peabody

Abstract

The coat proteins of the RNA bacteriophages Qbeta and MS2 are specific RNA binding proteins. Although they possess common tertiary structures, they bind different RNA stem loops and thus provide useful models of specific protein-RNA recognition. Although the RNA-binding site of MS2 coat protein has been extensively characterized previously, little is known about Qbeta. Here we describe the isolation of mutants that define the RNA-binding site of Qbeta coat protein, showing that, as with MS2, it resides on the surface of a large beta-sheet. Mutations are also described that convert Qbeta coat protein to the RNA binding specificity of MS2. The results of these and other studies indicate that, although they bind different RNAs, the binding sites of the two coat proteins are sufficiently similar that each is easily converted by mutation to the RNA binding specificity of the other.

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Citations

Aug 15, 2002·Archives of Biochemistry and Biophysics·Marc SpingolaDavid S Peabody
Jul 27, 2012·Biomacromolecules·Jason D FiedlerM G Finn
Sep 9, 2011·ACS Nano·Jolene L LauM G Finn
Feb 14, 1998·Nature Structural Biology·M A ConveryP G Stockley
Dec 11, 2007·Nature Structural & Molecular Biology·Jeffrey A ChaoRobert H Singer
Jul 15, 1997·Nucleic Acids Research·M Spingola, D S Peabody
Oct 5, 2002·Nucleic Acids Research·Francis Lim, David S Peabody
Sep 26, 2015·Journal of Molecular Biology·Tobias W Giessen, Pamela A Silver
Mar 3, 2018·Chemical Society Reviews·William M AumillerTrevor Douglas
May 25, 2002·The Journal of General Virology·J KlovinsJ van Duin
Aug 28, 1999·The Journal of Biological Chemistry·D S Peabody, A Chakerian
Apr 18, 2001·The Journal of Biological Chemistry·F LimD S Peabody
Sep 27, 2020·Applied and Environmental Microbiology·Joe Heffron, Brooke K Mayer
Oct 24, 2002·Archives of Biochemistry and Biophysics·Binnur Eroglu, Susan G Powers-Lee

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