The RNA component of human telomerase

Science
J FengJ Yu

Abstract

Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5'-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Jan 1, 1992·Developmental Genetics·A A AvilionC W Greider
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R C AllsoppC B Harley
Dec 1, 1992·American Journal of Obstetrics and Gynecology·J K Smith, G Yeh
Sep 1, 1992·Experimental Cell Research·J IrvingB Villeponteau
Jul 15, 1992·Cancer Genetics and Cytogenetics·D J AdamsonN E Haites
Feb 2, 1990·Science·D Shippen-Lentz, E H Blackburn
Mar 1, 1991·Mutation Research·C B Harley
Jan 1, 1991·Mutation Research·J LindseyH J Cooke
Feb 1, 1990·Molecular and Cellular Biology·T de LangeH E Varmus
May 31, 1990·Nature·C B HarleyC W Greider
Aug 30, 1990·Nature·N D HastieR C Allshire
Oct 18, 1972·Nature: New Biology·J D Watson
Mar 1, 1994·Genes & Development·C Autexier, C W Greider
Oct 21, 1994·Science·M S Singer, D E Gottschling
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·D BroccoliT de Lange
Aug 3, 1995·Nature·M J McEachern, E H Blackburn
Mar 1, 1993·Genes, Chromosomes & Cancer·K HolzmannW Henn
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·K R Prowse, C W Greider
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·H VaziriP M Lansdorp
Apr 1, 1994·Molecular and Cellular Biology·S K ChiouE White
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M CounterC B Harley
Dec 1, 1993·Cancer Genetics and Cytogenetics·H S SchwartzM G Butler
Feb 1, 1994·Molecular and Cellular Biology·A J KlingelhutzJ K McDougall
Aug 1, 1939·Proceedings of the National Academy of Sciences of the United States of America·B McClintock

❮ Previous
Next ❯

Citations

Nov 1, 1996·The Journal of Pathology·N J Royle
Nov 10, 1998·International Journal of Cancer. Journal International Du Cancer·T KanayaM Inoue
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·T SumidaN Ueda
Jul 15, 1999·International Journal of Cancer. Journal International Du Cancer·T W ParkF Jänicke
Sep 1, 1996·Journal of Cellular Physiology·M B SmallK A Sethi
Nov 20, 1997·Journal of Cellular Physiology·J W Shay
Jun 11, 1999·Journal of Cellular Physiology·S E Holt, J W Shay
Mar 10, 1998·Genes, Chromosomes & Cancer·B A Kosciolek, P T Rowley
Sep 17, 1998·Genes, Chromosomes & Cancer·H TanakaM Oshimura
Feb 27, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R R Reddel
Mar 27, 2001·International Journal of Cancer. Journal International Du Cancer·G B WismanA G van der Zee
Mar 27, 2001·International Journal of Cancer. Journal International Du Cancer·G A UlanerA R Hoffman
Mar 29, 2002·Arthritis and Rheumatism·Sonsoles Piera-VelazquezDavidG Stokes
Jan 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Holliday
Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Melek, D E Shippen
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T SudaH Asakura
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S OzakiY Nakanuma
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Rugang ZhangHong Xie
Jan 5, 2002·Journal of Surgical Oncology·Akira MitsuiYoshitaka Fujii
Oct 2, 2002·Journal of Surgical Oncology·Han-Jung ChenJui-Wei Lin
Dec 18, 2001·Journal of Neurobiology·G L Caporaso, M V Chao
Jun 16, 2001·The Journal of Pathology·P MatthewsD Wynford-Thomas
Nov 13, 2008·Cytotechnology·Kwang M LeeMichel M Ouellette
Sep 1, 1996·Somatic Cell and Molecular Genetics·C J Brown, S E Baldry
Mar 28, 2003·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yating TuZhixiang Liu
Jan 1, 1997·Pathology Oncology Research : POR·Adorján Aszalós, Sándor Eckhardt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.