The RNA Export Factor ALY1 Enables Genome-Wide RNA-Directed DNA Methylation.

The Plant Cell
Sarah G ChouduryR Keith Slotkin

Abstract

RNA-directed DNA methylation (RdDM) is a set of mechanisms by which transcriptionally repressive DNA and histone methylation are targeted to viruses, transposable elements, and some transgenes. We identified an Arabidopsis (Arabidopsis thaliana) mutant in which all forms of RdDM are deficient, leading to transcriptional activation of some transposable elements and the inability to initiate transgene silencing. The corresponding gene, ALY1, encodes an RNA binding nuclear export protein. Arabidopsis ALY proteins function together to export many messenger RNAs (mRNAs), but we found that ALY1 is unique among this family for its ability to enable RdDM. Through the identification of ALY1 direct targets via RNA immunoprecipitation sequencing, coupled with mRNA sequencing of nuclear and cytoplasmic fractions, we identified mRNAs of known RdDM factors that fail to efficiently export from the nucleus in aly1 mutants. We found that loss of RdDM in aly1 is a result of deficient nuclear export of the ARGONAUTE6 mRNA and subsequent decreases in ARGONAUTE6 protein, a key effector of RdDM. One aly1 allele was more severe due to an additional loss of RNA Polymerase V function, which is also necessary for RdDM. Together, our data reconcile the b...Continue Reading

Citations

Apr 12, 2019·Journal of Experimental Botany·Hans F EhrnsbergerKlaus D Grasser
Aug 16, 2019·Molecular Plant-microbe Interactions : MPMI·Min XuRichard Kormelink
Aug 8, 2020·Frontiers in Plant Science·Francesca MaioHarrold A van den Burg
Nov 24, 2021·Molecular Biology Reports·Emre AksoyM Aydın Akbudak

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