The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing

Genes & Development
Anastassios VourekasP Jeremy Wang

Abstract

Piwi-piRNA (Piwi-interacting RNA) ribonucleoproteins (piRNPs) enforce retrotransposon silencing, a function critical for preserving the genome integrity of germ cells. The molecular functions of most of the factors that have been genetically implicated in primary piRNA biogenesis are still elusive. Here we show that MOV10L1 exhibits 5'-to-3' directional RNA-unwinding activity in vitro and that a point mutation that abolishes this activity causes a failure in primary piRNA biogenesis in vivo. We demonstrate that MOV10L1 selectively binds piRNA precursor transcripts and is essential for the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Multiple analyses suggest an intimate coupling of piRNA precursor processing with elements of local secondary structures such as G quadruplexes. Our results support a model in which MOV10L1 RNA helicase activity promotes unwinding and funneling of the single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing.

References

Nov 5, 1997·Nature Genetics·M Lewandoski, G R Martin
Jan 23, 2004·Development·Satomi Kuramochi-MiyagawaToru Nakano
Jul 16, 2004·Molecular and Cellular Biology·Alexei A AravinVladimir A Gvozdev
Jun 6, 2006·Nature·Angélique GirardMichelle A Carmell
Jun 6, 2006·Nature·Alexei AravinThomas Tuschl
Jun 13, 2006·Genes & Development·Shane T GrivnaHaifan Lin
Jun 17, 2006·Science·Nelson C LauRobert E Kingston
Jul 1, 2006·Science·Vasily V VaginPhillip D Zamore
Mar 27, 2007·Nature Structural & Molecular Biology·Yohei Kirino, Zissimos Mourelatos
Mar 31, 2007·Developmental Cell·Michelle A CarmellGregory J Hannon
Apr 21, 2007·Science·Alexei A AravinGregory J Hannon
Jul 27, 2007·RNA·Yohei Kirino, Zissimos Mourelatos
Jan 17, 2009·Nature Reviews. Genetics·Megha Ghildiyal, Phillip D Zamore
Apr 21, 2009·Nature Cell Biology·Yohei KirinoZissimos Mourelatos
Jun 19, 2009·Nature·Sung Wook ChiRobert B Darnell
Dec 22, 2009·Current Biology : CB·Nicolas RobineEric C Lai
May 12, 2010·Current Opinion in Structural Biology·Margaret E Fairman-WilliamsEckhard Jankowsky
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ke ZhengPeijing Jeremy Wang
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert J A FrostEric N Olson
Jun 24, 2010·Nucleic Acids Research·Jean-Denis Beaudoin, Jean-Pierre Perreault
Sep 23, 2010·The EMBO Journal·Leonie M KammingaRené F Ketting
Oct 23, 2010·Genes & Development·Kuniaki SaitoMikiko C Siomi
Oct 23, 2010·Genes & Development·Astrid D HaaseGregory J Hannon
Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Mikiko C SiomiAlexei A Aravin
Sep 20, 2011·Molecular Cell·Shinpei KawaokaYukihide Tomari

❮ Previous
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Citations

Jan 27, 2016·Trends in Biochemical Sciences·Benjamin Czech, Gregory J Hannon
Oct 31, 2015·Molecular Reproduction and Development·David H TaylorPaul D Soloway
Mar 8, 2016·Nature·Anastassios VourekasZissimos Mourelatos
Mar 10, 2016·Seminars in Cell & Developmental Biology·Fang Yang, P Jeremy Wang
Nov 20, 2015·Seminars in Cell & Developmental Biology·Robyn S M Lim, Toshie Kai
Sep 12, 2015·Developmental Biology·Alix Luangpraseuth-ProsperBéatrice Mandon-Pépin
Jun 10, 2016·Clinical Epigenetics·Andrew Y F Li YimPeter Henneman
Aug 16, 2016·Mobile DNA·John L Goodier
Jun 21, 2017·Proceedings of the National Academy of Sciences of the United States of America·Simon J NewkirkWenfeng An
Mar 7, 2018·Nature Structural & Molecular Biology·Fadia IbrahimZissimos Mourelatos
Sep 9, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Didier Auboeuf
Oct 19, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Tiina Lehtiniemi, Noora Kotaja
Nov 27, 2018·Annual Review of Genetics·Benjamin CzechGregory J Hannon
Dec 21, 2018·Genes & Development·Chenchun WengEric A Miska
Jan 18, 2019·Non-coding RNA·Miguel Vasconcelos AlmeidaRené F Ketting
Aug 2, 2019·The EMBO Journal·Kensaku MuranoHaruhiko Siomi
Feb 14, 2018·EMBO Reports·Takuji YoshimuraJun-Ichi Miyazaki
Sep 13, 2019·Wiley Interdisciplinary Reviews. RNA·Prakash KharelSoumitra Basu
Nov 2, 2018·BMC Evolutionary Biology·Andrew D CalcinoBernard M Degnan
Mar 7, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Gong-Wei ZhangFuyuan Zuo
May 10, 2020·Genes & Development·Fang YangP Jeremy Wang
Oct 18, 2019·The Journal of Infectious Diseases·Annabelle MouammineLouis Flamand
Nov 18, 2018·Nature Reviews. Genetics·Deniz M OzataPhillip D Zamore
Jul 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·P Jeremy Wang
Nov 12, 2017·Nature Communications·Christina ErnstClaudia Kutter
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·S J RussellJ LaMarre
Feb 6, 2020·Nature Cell Biology·Yu H SunXin Zhiguo Li
Feb 6, 2020·Nature Cell Biology·Yuanhui Mao, Shu-Bing Qian
Aug 9, 2020·Nucleic Acids Research·Deqiang DingChen Chen
Apr 25, 2020·Journal of Applied Genetics·Katarzyna Tutak, Natalia Rozwadowska
May 3, 2019·Wiley Interdisciplinary Reviews. RNA·Luis María Vaschetto, Hernán Mario Beccacece
Jul 30, 2019·National Science Review·Haixin LiEugene Yujun Xu
Jan 31, 2020·Nature·Natsuko IzumiYukihide Tomari
May 17, 2017·International Journal of Molecular Sciences·Valeria SpecchiaMaria Pia Bozzetti

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