The RNA polymerase-binding protein RbpA confers basal levels of rifampicin resistance on Streptomyces coelicolor.

Molecular Microbiology
Katy V NewellMark S B Paget

Abstract

RbpA is an RNA polymerase-binding protein that occurs in the actinomycete family of bacteria and is regulated by the disulphide stress-response sigma factor, sigma(R), in Streptomyces coelicolor. Here we demonstrate that rbpA null mutants exhibit a slow-growth phenotype and are particularly sensitive to the transcription inhibitor rifampicin. Strikingly, transcription mapping experiments revealed that rbpA expression is induced upon exposure of S. coelicolor to rifampicin and that this, in part, involves an increase in the activity of sigma(R). In contrast, the ribosomal RNA operon promoter rrnDp3, which is recognized by the vegetative sigma factor sigma(HrdB), was strongly inhibited by rifampicin. Reconstitution of RNAP from an rbpA null mutant with purified RbpA revealed that RbpA stimulates transcription from rrnDp3, even in the presence of rifampicin. The data presented suggest that RbpA confers basal levels of rifampicin resistance and is a novel regulator of rRNA synthesis in S. coelicolor.

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Citations

Aug 2, 2007·Journal of Applied Genetics·Agnieszka Szalewska-PalaszAlicja Wegrzyn
Apr 23, 2013·Nucleic Acids Research·Aline Tabib-SalazarMark S Paget
Jun 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A HubinMark S Paget
Mar 11, 2008·Biochemical and Biophysical Research Communications·Raju Mukherjee, Dipankar Chatterji
Mar 13, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P SinghS T Cole
Apr 1, 2009·Microbiology·Roberta ProvvediRiccardo Manganelli
Feb 27, 2010·International Journal of Antimicrobial Agents·Audrey TupinJean-Paul Leonetti
Oct 30, 2018·Nucleic Acids Research·Klára ŠmídováJiri Vohradsky
Dec 28, 2019·Transcription·Hande BoyaciElizabeth A Campbell
Aug 28, 2020·Frontiers in Microbiology·Rishi Kishore Vishwakarma, Konstantin Brodolin
Dec 19, 2019·Scientific Reports·Phani Rama Krishna BehraLeif A Kirsebom
Apr 4, 2013·The Journal of Biological Chemistry·Alessio BortoluzziHelen M O'Hare
Jun 24, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Subho Ghosh, Dipankar Chatterji
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Gundeep KaurKrishan Gopal Thakur
Feb 20, 2021·Proceedings of the National Academy of Sciences of the United States of America·Marie DelabyPatrick H Viollier
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Sep 4, 2020·Microbiological Research·Paolo TurriniPaolo Visca
May 26, 2012·Applied & Translational Genomics·Abhinav DeyDipankar Chatterji

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