The role and possible mechanism of long noncoding RNA PVT1 in modulating 3T3-L1 preadipocyte proliferation and differentiation.

IUBMB Life
Lin ZhangZi-Yang Shen

Abstract

Obesity is considered as a high-risk susceptibility state for most metabolic disorders and is directly related to preadipocyte differentiation or adipogenesis. Long noncoding RNAs (lncRNAs) are the key factors which have regulatory functions on various critical physiological and biological processes. PVT1 was identified as an oncogenic lncRNA which could promote angiogenesis in gastric cancer. However, the functions and molecular pathways related to PVT1 in adipogenesis had not been clarified yet. In the current study, the purpose was to identify the effects of lncRNA PVT1 on adipogenesis and the relevant molecular processes. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to quantify PVT1 expression. The mechanism for PVT1 to participate in 3T3-L1 adipogenesis was identified by lentivirus-mediated gain- and loss-of-function tests. The potential association of PVT1 with cell viability was checked by CCK-8 assay and EdU staining. The gene expression for cytokines was determined by quantitative PCR (qPCR) and western blotting. PVT1 expression level was strongly upregulated after 3T3-L1 preadipocytes differentiated. In mice, PVT1 was abundantly expressed in adipose tissue, and the obese mice had higher PVT1 exp...Continue Reading

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Citations

Jul 31, 2020·ELife·Tiziana SquillaroGiovanni Di Bernardo
Sep 29, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Songjia LaiShiyi Chen
Dec 22, 2020·European Journal of Pharmacology·Soudeh Ghafouri-Fard, Mohammad Taheri
Jan 15, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Federica ReyStephana Carelli

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