The Role and Underlying Mechanism of Exosomal CA1 in Chemotherapy Resistance in Diffuse Large B Cell Lymphoma

Molecular Therapy. Nucleic Acids
Yuhua FengHui Zhou

Abstract

Chemotherapy resistance plays a major role in treatment failure of diffuse large B cell lymphoma (DLBCL). Exosomes are closely related to tumor drug resistance. Herein, the expression of exosomal proteins in DLBCL and their roles in chemotherapy resistance of DLBCL are explored. Tandem mass tag labeling proteomics was used to perform proteomic profiling in exosomes from DLBCL patients' serum. The expression of carbonic anhydrase 1 (CA1) in parental, chemo-resistant DLBCL cells and DLBCL patient exosomes was detected. Proliferation of DLBCL following CA1 knockdown was investigated both in vitro and in vivo, along with the effects on nuclear factor κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) pathways. We identified 54 differentially expressed proteins. We validated that the expression level of exosomal CA1 was higher in chemo-resistant DLBCL cells than in chemo-sensitive counterparts. Knockdown of CA1 inhibited the growth of DLBCL via inhibiting the activation of NF-κB and STAT3 signaling pathways both in vitro and in vivo. An increased expression level of exosomal CA1 was associated with poorer prognosis, and exosomal CA1 could be used as a biomarker to predict chemotherapeutic efficacy. Our study s...Continue Reading

Citations

Dec 29, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Yu-Shui MaDa Fu
Apr 6, 2021·Frontiers in Oncology·Liwei Lv, Yuanbo Liu
Nov 9, 2021·Mass Spectrometry Reviews·Iqbal JalaludinJeongkwon Kim

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Datasets Mentioned

BETA
PXD019780

Methods Mentioned

BETA
dynamic
transmission electron microscopy
proteomics profiling
transfection
xenograft
proteomic profiling

Software Mentioned

GraphPad Prism
SPSS

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