PMID: 6109228Jan 1, 1980

The role fo glutamine synthetase and glutamine metabolism in nitrogen metabolite repression, a regulatory phenomenon in the lower eukaryote Neurospora crassa

Molecular & General Genetics : MGG
N S Dunn-Coleman, R H Garrett


Growth of Neurospora crassa on media containing NH4+ leads to the repression of a variety of permeases and alternative pathways which would generate NH4+, so called "ammonium repression." The mutant am2 which lacks NADP-GDH is not subject to ammonium repression of nitrate reductase or urea permease, but like the wild type has repressed levels of these systems when grown in the presence of proline, glutamate or glutamine. The glutamine synthetase (GS) mutant gln-1a has derepressed levels of the aforementioned systems unless grown with glutamine. The oligomeric state of GS depends upon the nitrogen sufficiency of the cell, a tetrameric form predominates under conditions of nitrogen limitation and an octameric form under conditions of nitrogen sufficiency. We have found that the tetrameric form GS predominates in the mutants am2 and gln-1a when they are ammonium derepressed. Th mechanism of NH4+ repression in N. crassa is thought to entail a cessation of positive gene action by the product of the nit-2 regulatory gene. We propose that under conditions of NH4+ sufficiency, and hence glutamine sufficiency, the octameric form of GS represses nit-2 gene expression and thereby achieves ammonium repression.


Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E GarciaS Kustu
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Jan 1, 1981·Molecular & General Genetics : MGG·N S Dunn-ColemanR H Garrett
Jan 1, 1982·Molecular & General Genetics : MGG·F P Buxton, A Radford
Oct 1, 1982·Current Genetics·J A Chambers, S A Wilkins
Jan 1, 1984·Current Genetics·E V Cornwell, D W Macdonald
Aug 1, 1982·Biochemical Genetics·J A PatemanE M Mackay
Jan 1, 1983·Biotechnology Advances·R Hütter, P Niederberger
Oct 11, 2005·FEMS Yeast Research·Beatrice RossiEnrico Berardi
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Sep 22, 2001·Journal of Bacteriology·S MargelisM A Davis
Dec 17, 2014·Frontiers in Microbiology·Bettina Tudzynski
Nov 17, 2017·Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine·Mohandeep KaurJyoti Sharma

Related Concepts

Quaternary Ammonium Compounds
Enzyme Repression
Genes, Regulator
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Neurospora crassa

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