The role of 2,4-dihydroxyquinoline (DHQ) in Pseudomonas aeruginosa pathogenicity

PeerJ
Jordon D GruberYong-Mei Zhang

Abstract

Bacteria synchronize group behaviors using quorum sensing, which is advantageous during an infection to thwart immune cell attack and resist deleterious changes in the environment. In Pseudomonas aeruginosa, the Pseudomonas quinolone signal (Pqs) quorum-sensing system is an important component of an interconnected intercellular communication network. Two alkylquinolones, 2-heptyl-4-quinolone (HHQ) and 2-heptyl-3-hydroxy-4-quinolone (PQS), activate transcriptional regulator PqsR to promote the production of quinolone signals and virulence factors. Our work focused on the most abundant quinolone produced from the Pqs system, 2,4-dihydroxyquinoline (DHQ), which was shown previously to sustain pyocyanin production and antifungal activity of P. aeruginosa. However, little is known about how DHQ affects P. aeruginosa pathogenicity. Using C. elegans as a model for P. aeruginosa infection, we found pqs mutants only able to produce DHQ maintained virulence towards the nematodes similar to wild-type. In addition, DHQ-only producing mutants displayed increased colonization of C. elegans and virulence factor production compared to a quinolone-null strain. DHQ also bound to PqsR and activated the transcription of pqs operon. More importantl...Continue Reading

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Citations

Dec 4, 2019·Journal of Medical Microbiology·Selene García-ReyesMiguel Cocotl-Yanez
Nov 10, 2018·Beilstein Journal of Organic Chemistry·Christian Schütz, Martin Empting
Jul 23, 2020·Food & Function·Nicholas MokSong Lin Chua
Oct 9, 2018·Frontiers in Microbiology·Wei ChenChristopher Davies
Dec 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad SaalimBenjamin R Clark
Feb 20, 2019·Journal of Proteome Research·Troy D HubbardGary H Perdew

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Methods Mentioned

BETA
PCR
gene knockout
X-ray
NMR
electrophoretic mobility shift

Software Mentioned

ImageJ
MASS LYNX
GraphPad Prism

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