The role of actin-binding protein 280 in integrin-dependent mechanoprotection.

The Journal of Biological Chemistry
M GlogauerChristopher A McCulloch

Abstract

To survive in a mechanically active environment, cells must adapt to variations of applied membrane tension. A collagen-coated magnetic bead model was used to apply forces directly to the actin cytoskeleton through integrin receptors. We demonstrate here that by a calcium-dependent mechanism, human fibroblasts reinforce locally their connection with extracellular adhesion sites by inducing actin assembly and by recruiting actin-binding protein 280 (ABP-280) into cortical adhesion complexes. ABP-280 was phosphorylated on serine residues as a result of force application. This phosphorylation and the force-induced actin reorganization were largely abrogated by inhibitors of protein kinase C. In a human melanoma cell line that does not express ABP-280, actin accumulation could not be induced by force, whereas in stable transfectants expressing ABP-280, force-induced actin accumulation was similar to human fibroblasts. Cortical actin assembly played a role in regulating the activity of stretch-activated, calcium-permeable channels (SAC) since sustained force application desensitized SAC to subsequent force applications, and the decrease in stretch sensitivity was reversed after treatment with cytochalasin D. ABP-280-deficient cells ...Continue Reading

References

Jan 1, 1992·Cell Motility and the Cytoskeleton·L W JansonD L Taylor
Sep 1, 1991·The Journal of Cell Biology·L W JansonD L Taylor
Feb 1, 1991·Current Opinion in Cell Biology·J H Hartwig, D J Kwiatkowski
Sep 1, 1990·The Journal of Cell Biology·J B GorlinJ H Hartwig
Jan 1, 1985·The Journal of Cell Biology·J R OkitaT J Kunicki
Jun 15, 1993·Biochemical and Biophysical Research Communications·G Plopper, D E Ingber
Jan 27, 1995·Cell·D E Clapham
Jun 1, 1993·Journal of Cellular Physiology·C A McCulloch, G C Knowles
Oct 1, 1995·Kidney International·H F Cantiello
Sep 1, 1996·Journal of Cellular Physiology·W LeeC A McCulloch
Apr 7, 1997·The Journal of Cell Biology·P L McNeil, R A Steinhardt
Feb 7, 1998·Pflügers Archiv : European journal of physiology·M Glogauer, J Ferrier
Sep 1, 1993·Trends in Cell Biology·P L McNeil

❮ Previous
Next ❯

Citations

Sep 10, 2003·Analytical Biochemistry·Edzard SpillnerReinhard Bredehorst
Feb 19, 2000·Current Opinion in Cell Biology·D R Critchley
Apr 6, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Maria Lonnett BurgessThomas K Borg
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Céline RevenuDaniel Louvard
Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Scott SiechenTaher Saif
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lorenz RognoniMatthias Rief
Jan 15, 2010·The Journal of Biological Chemistry·Matthew W C ChanChristopher A McCulloch
Jun 14, 2008·The Journal of Biological Chemistry·Baldwin C MakChristopher A McCulloch
Jan 16, 2009·Molecular Biology of the Cell·Yulia ShifrinChristopher A McCulloch
May 22, 2009·Molecular Biology of the Cell·Scott GehlerPatricia J Keely
Oct 7, 2009·Molecular Biology and Evolution·Barry A KesnerNikolay V Dokholyan
Apr 28, 2007·Annual Review of Biomedical Engineering·Paul A Janmey, Christopher A McCulloch
Feb 8, 2006·Annual Review of Physiology·Jeffrey J Fredberg, Roger D Kamm
Oct 31, 2002·American Journal of Respiratory and Critical Care Medicine·Nicholas E VlahakisRolf D Hubmayr
Nov 14, 2007·BMC Cell Biology·Sarah J AnnesleyPaul R Fisher
Feb 8, 2013·Cell & Bioscience·Jingyin YueZhiyuan Shen
May 9, 2009·PloS One·Ramaswamy KrishnanJeffrey J Fredberg
Jan 1, 2009·Archives of Histology and Cytology·Takeomi MizutaniKazushige Kawabata
Jan 5, 2013·International Journal of Biological Sciences·Xi JiangZhiyuan Shen
May 2, 2008·The Review of Scientific Instruments·Nathan J SniadeckiDaniel H Reich
Mar 13, 2012·Annual Review of Biophysics·Ziba RaziniaDavid A Calderwood
Aug 22, 2014·Molecular Biology of the Cell·Lukas D OsborneKarthikeyan Mythreye
Feb 13, 2002·The Journal of Cell Biology·Yasuhiro Sawada, Michael P Sheetz
Jan 12, 2000·The Journal of Cell Biology·T G ThompsonL M Kunkel
Nov 26, 2010·FEBS Letters·Hugh Kim, Christopher A McCulloch
Oct 12, 2001·Journal of Cellular Biochemistry·N CrosC A Dechesne
Jan 12, 2010·Trends in Cell Biology·Alex-Xianghua ZhouLevent M Akyürek

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.