The role of adhesion molecules in the recruitment of hepatic natural killer cells (pit cells) in rat liver

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
D LuoE Wisse

Abstract

Previous studies showed that blood large granular lymphocytes (LGL), which possess natural killer (NK) activity, develop within rat liver sinusoids into high-density (HD) and subsequently into low-density (LD) pit cells which show an increasing level and spectrum of tumor cytotoxicity. In this study, we investigated the role of adhesion molecules, such as CD2, CD11a, CD18, and CD54 in the recruitment of pit cells to the liver. Immunostaining for electron microscopy, and two color flow cytometry showed that most pit cells expressed CD2, CD11a, CD18, and CD54. After intravenous injections into rats with anti-CD2, anti-CD11a, and anti-CD18 antibodies, the number of pit cells per square millimeter in frozen sections of liver tissue decreased. Treatment of rats with zymosan increased the number of pit cells fivefold, whereas subsequent treatment with anti-adhesion-molecule antibodies resulted in approximately 60% lower number of pit cells. Anti-CD54, supposed to block CD54 expression on sinusoidal endothelial cells, also decreased the number of pit cells. The number of blood LGL was, however, not affected by these antibodies. These results indicate that blocking of CD2, CD11a, CD18, and CD54 antigens on blood LGL and/or liver endoth...Continue Reading

Citations

Jun 7, 2007·Histochemistry and Cell Biology·Albrecht StenzingerMonika Wimmer
Dec 29, 1998·Immunology Today·A RubartelliM R Zocchi
Jul 25, 2012·World Journal of Gastroenterology : WJG·Jie YangJiao Liu
Apr 20, 2014·World Journal of Gastroenterology : WJG·Konstantinos A PaschosNigel C Bird
Jul 25, 2013·Journal of Autoimmunity·Kelly HudspethDomenico Mavilio
Feb 10, 2016·Frontiers in Immunology·Frank FasbenderCarsten Watzl
May 23, 2019·Frontiers in Immunology·Joanna MikulakDomenico Mavilio
Aug 30, 2005·World Journal of Gastroenterology : WJG·Katrien Vekemans, Filip Braet

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