The role of adipose-derived stem cells in a self-organizing 3D model with regard to human soft tissue healing

Molecular and Cellular Biochemistry
Martin OberringerTim Pohlemann

Abstract

The clinical phenomenon of inadequate soft tissue healing still remains an important issue. The occurrence of chronic wounds is correlated to the life span, which is still increasing in western countries. Tissue engineering products containing adipose-derived stem cells are discussed as a promising therapeutic approach. Several studies confirmed the value of these cells for soft tissue healing improvement, suggesting a paracrine as well as a direct effect on vessel repair and angiogenesis. In an attempt to figure out specific effects of adipose-derived stem cells on dermal microvascular endothelial cells with respect to the different phases of soft tissue healing, we designed a 3D in vitro model on the basis of spheroids. Basic parameters like spheroid volume, cell numbers, and rate of apoptotic cells were determined in dependence on culture time, on different oxygen conditions and using mono- as well as co-cultures of both cell types. Furthermore we focused on gene expression and protein levels of interleukin-6, interleukin-8, monocyte chemoattractant protein-1, and vascular endothelial growth factor, which are discussed against the background of therapies for chronic wounds. The visualization of α-smooth muscle actin allowed ...Continue Reading

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Citations

Jan 18, 2020·Artificial Organs·Gabriela S KronembergerLeandra Santos Baptista
Sep 5, 2020·Biological Procedures Online·Cheng-Li LinTing-Yuan Tu
Jan 21, 2021·International Journal of Biological Macromolecules·F ToiaC Dispenza

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Methods Mentioned

BETA
light microscopy
PCR
Assay
enzyme-linked immunosorbent assay

Software Mentioned

[UNK]
AxioVision
NormFinder
qlMP
qlMF

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