The role of an ancestral hyperpolarization-activated cyclic nucleotide-gated K+ channel in branchial acid-base regulation in the green crab, Carcinus maenas

The Journal of Experimental Biology
Sandra Fehsenfeld, Dirk Weihrauch

Abstract

Numerous electrophysiological studies on branchial K(+) transport in brachyuran crabs have established an important role for potassium channels in osmoregulatory ion uptake and ammonia excretion in the gill epithelium of decapod crustaceans. However, hardly anything is known of the actual nature of these channels in crustaceans. In the present study, the identification of a hyperpolarization-activated cyclic nucleotide-gated potassium channel (HCN) in the transcriptome of the green crab Carcinus maenas and subsequent performance of quantitative real-time PCR revealed the ubiquitous expression of this channel in this species. Even though mRNA expression levels in the cerebral ganglion were found to be approximately 10 times higher compared with all other tissues, posterior gills still expressed significant levels of HCN, indicating an important role for this transporter in branchial ion regulation. The relatively unspecific K(+)-channel inhibitor Ba(2+), as well as the HCN-specific blocker ZD7288, as applied in gill perfusion experiments and electrophysiological studies employing the split gill lamellae revealed the presence of at least two different K(+)/NH4(+)-transporting structures in the branchial epithelium of C. maenas. F...Continue Reading

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Citations

Jan 24, 2018·The Journal of Experimental Biology·Stephanie HansDirk Weihrauch
Apr 26, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Čedomil Lucu
Jan 31, 2018·The Journal of Experimental Biology·Dirk WeihrauchGarett J P Allen
Feb 9, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Garett Joseph Patrick Allen, Dirk Weihrauch
Jan 14, 2020·Respiratory Physiology & Neurobiology·Junho EomChris M Wood
Feb 21, 2021·Current Opinion in Insect Science·Dirk Weihrauch, Michael J O'Donnell

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