PMID: 2226470Oct 24, 1990

The role of an invariant tryptophan residue in alpha-bungarotoxin and cobrotoxin. Investigation of active derivatives with the invariant tryptophan replaced by kynurenine

European Journal of Biochemistry
C C ChangK Hayashi

Abstract

Ozone oxidation converted the single, invariant, tryptophan residue to N2-formylkynurenine in alpha-bungarotoxin and cobrotoxin. Upon this modification, the lethal toxicity was significantly reduced in cobrotoxin but mostly retained in alpha-bungarotoxin. Each neurotoxin containing kynurenine instead of tryptophan retained the same antigenicity as the native toxin. Fluorescence and CD spectroscopy revealed that, although the environment and state of the kynurenine residue were similar, [Kyn29]cobrotoxin was much more sensitive to pH change than alpha-[Kyn28]bungarotoxin. In terms of lethal toxicity and conformational stability, the invariant tryptophan residue appears to play a more important role in cobrotoxin, imparting a higher lethal toxicity than that in alpha-bungarotoxin, which has a disulfide bond at Cys29-Cys33.

References

May 3, 1977·Biochemistry·Y H ChenJ T Yang
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·L Y ChuangC C Chang
Dec 16, 1985·European Journal of Biochemistry·P E BourneB W Low
Feb 21, 1973·Biochimica Et Biophysica Acta·C C Chang, C C Yang
Apr 23, 1974·Biochemical and Biophysical Research Communications·M Ota, K Hayashi
Jan 1, 1972·Annual Review of Pharmacology·C Y Lee
Dec 6, 1973·Biochimica Et Biophysica Acta·E KarlssonH Drevin
Sep 29, 1970·Biochimica Et Biophysica Acta·A SetoN Tamiya
Apr 27, 1971·Biochimica Et Biophysica Acta·C C ChangK Hayashi
Feb 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·D MebsC Y Lee
Aug 6, 1971·Biochemical and Biophysical Research Communications·D MebsC Y Lee
Nov 20, 1969·Biochemical and Biophysical Research Communications·C C Chang, K Hayashi
Jun 1, 1969·Toxicon : Official Journal of the International Society on Toxinology·C C YangT Suzuki
Jan 1, 1983·CRC Critical Reviews in Biochemistry·M J Dufton, R C Hider
Jul 1, 1982·Journal of Biochemistry·Y Fukunaga, F Sakiyama

Related Concepts

Antibody Formation
kappa-Bungarotoxin
Circular Dichroism, Vibrational
Cobrotoxin
Kynurenine
Precipitin Tests
Snakes
Fluorescence Spectroscopy
PMS-Tryptophan

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