Dec 8, 1978

The role of arginine residues in the function of D-glyceraldehyde-3-phosphate dehydrogenase

Biochimica Et Biophysica Acta
N K NagradovaN V Benkevich

Abstract

Inactivation of apo-glyceraldehyde-3-phosphate dehydrogenase from rat skeletal muscle in the presence of butanedione is the result of modification of one arginyl residue per subunit of the tetrameric enzyme molecule. The loss of activity follows pseudo-first-order kinetics. NAD+ increases the apparent first-order rate constant of inactivation. The effect of NAD+ on the enzyme inactivation is cooperative (Hill coefficient = 2.3--3.2). Glyceraldehyde 3-phosphate protected the holoenzyme against inactivation, decreasing the rate constant of the reaction. At saturating concentrations of substrate the protection was complete. The Hill plot demonstrates that the effect is cooperative. This suggests that subunit interactions in the tetrameric holoenzyme molecule may affect the reactivity of the essential arginyl residues. In contrast, glyceraldehyde 3-phosphate had no effect on the rate of inactivation of the apoenzyme in the presence of butanedione. 100 mM inorganic phosphate protected both the apoenzyme and holoenzyme against inactivation. The involvement of the microenvironment of the arginyl residues in the functionally important conformational changes of the enzyme is discussed.

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Mentioned in this Paper

Arginine hydrochloride
Macromolecular Compounds
Apoenzymes
Pseudo brand of pseudoephedrine
Neutrophil Actin Dysfunction
GAPDH gene
Phosphate Measurement
NADH
Glyceraldehyde
Rat Skeletal Muscle

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