The role of Beclin 1 in SDT-induced apoptosis and autophagy in human leukemia cells

International Journal of Radiation Biology
Xiaomin SuAlbert Wingnang Leung

Abstract

To prove the occurrence of autophagy after treatment by protoporphyrin IX (PpIX)-mediated sonodynamic therapy (SDT) of human chronic myelogenous leukemia K562 cells as well as its relationship with apoptosis. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylter-trazolium bromide tetrazolium (MTT) assay was adopted to examine cytotoxicity of different treatments. Nuclear morphology changes were observed under a fluorescence microscopy with 4'-6-Diamidino-2-Phenylindole (DAPI) staining. Western blotting was used to analyze the expression of caspase-3, Beclin 1 (BECN 1) and the conversion of LC3- phosphatidylethanolamine conjugate/a cytosolic form of LC3 (LC3 II/I). Fluorescence microscope was used to identify the formation of autophagic vacuoles (AVO) during autophagy. Under optimal conditions, SDT was shown to induce autophagy in K562 cells, which caused the up-regulation of Beclin-1 and the formation of AVO. In addition, pre-treatment of cancer cells with Beclin 1-targeted short hairpin RNA (Beclin 1 shRNA) was shown to reduce the level of LC3-II accumulation and staining with punctate spots of monodansylcadaverine (MDC) staining. Besides, the cytotoxic effect of SDT was significantly increased by Beclin 1 shRNA. Furthermore, stu...Continue Reading

References

Nov 26, 2003·The Journal of Clinical Investigation·Xueping QuBeth Levine
Nov 5, 2004·Molecular and Cellular Biochemistry·Ana Maria Cuervo
Apr 30, 2005·The Plant Journal : for Cell and Molecular Biology·Anthony L ContentoDiane C Bassham
Jul 29, 2006·Autophagy·Yasuko Kondo, Seiji Kondo
Mar 22, 2007·Archives of Biochemistry and Biophysics·Elisabetta Ferraro, Francesco Cecconi
Aug 25, 2007·Nature Reviews. Molecular Cell Biology·M Chiara MaiuriGuido Kroemer
Oct 12, 2007·Biochimie·Sophie PattingrePatrice Codogno
Oct 18, 2007·The Journal of Biological Chemistry·Alejandro RopoloMaria I Vaccaro
Apr 25, 2008·Ultrasonics·Katsuro TachibanaYurika Ikeda-Dantsuji
Jun 3, 2008·Ultrasonics Sonochemistry·Quanhong LiuWei Tang
Oct 22, 2008·Ultrasonics·Jhony El MaaloufJean-Louis Mestas
Feb 27, 2009·Ultrasound in Medicine & Biology·Na MiBing Cao
Mar 28, 2009·Cell Death and Differentiation·A Eisenberg-LernerA Kimchi
Jan 26, 2010·Current Opinion in Cell Biology·Congcong He, Beth Levine
Mar 10, 2010·Ultrasound in Medicine & Biology·Xiao Bing WangXiu Juan Li
Mar 13, 2010·Biochemical and Biophysical Research Communications·Yan-Lin ZhangChun-Feng Liu
Oct 5, 2010·Ultrasound in Medicine & Biology·Xiaobing WangYixiang Li
Jan 29, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shubham ShrivastavaRatna B Ray
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
May 5, 2011·Cancer Biotherapy & Radiopharmaceuticals·Ping ZhaoMengying Zhang
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Nov 16, 2012·Journal of Lipid Research·Megan M YoungHong-Gang Wang
Dec 1, 2012·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Pan WangQuanhong Liu
Jan 3, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xiaobing WangQuanhong Liu
May 1, 2013·International Journal of Medical Sciences·Jing hua YuXiao fang Yu
Aug 1, 2004·Molecular and Cellular Biochemistry·Ana Maria Cuervo

❮ Previous
Next ❯

Citations

Sep 10, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Zhen-Tao MoQi-Hai Gong
Jan 20, 2017·Cell Death & Disease·Jiayuan Y KouLiming M Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Related Papers

The International Journal of Biochemistry & Cell Biology
Xiaomin SuXiaobing Wang
Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus
Y S ChenT Fu
Toxicology in Vitro : an International Journal Published in Association with BIBRA
Xiaobing WangQuanhong Liu
© 2022 Meta ULC. All rights reserved