The role of beta 1 integrins in adhesion of two breast carcinoma cell lines to a model endothelium

Clinical & Experimental Metastasis
R D BlissT W Lennard

Abstract

Interactions between tumour cells and the endothelium are vital to the formation of haematogenous metastases. Binding to model endothelium of one oestrogen receptor positive breast carcinoma cell line (MCF-7) and one receptor negative line (HS578T) was examined in vitro together with endothelial retraction induced by these tumour cells. Adhesion was inhibited by monoclonal antibodies specific for the VLA integrins and by peptides containing the RGD motif which is commonly recognised as a ligand by the VLA adhesion molecules. However, binding of the two tumour cell lines was inhibited by monoclonal antibodies specific for different VLA molecules; anti-alpha 6 beta 1 inhibited MCF-7 adhesion but anti-alpha 5 beta 1 inhibited Hs578T. These results were consistent with flow cytometric quantification of the expression of these VLA integrins on the surfaces of the two tumour cell lines. Enzyme-linked immunosorbent assays (ELISA) demonstrated that laminin was present on the endothelial cell surface but collagen IV was absent. ELISA failed to detect increased exposure of the subendothelial matrix during the first hour after addition of either cancer cell type. This was supported by assays which demonstrated maintenance of the endotheli...Continue Reading

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Citations

Jul 27, 2012·The Journal of Biological Chemistry·Mehran HaidariRichard A F Dixon
Sep 15, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·S ArtikT Ruzicka
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