The role of biological fluid and dynamic flow in the behavior and cellular interactions of gold nanoparticles

Journal of Nanobiotechnology
Emily K BreitnerKristen K Comfort

Abstract

Due to their distinctive physicochemical properties, nanoparticles (NPs) have proven to be extremely advantageous for product and application development, but are also capable of inducing detrimental outcomes in biological systems. Standard in vitro methodologies are currently the primary means for evaluating NP safety, as vast quantities of particles exist that require appraisal. However, cell-based models are plagued by the fact that they are not representative of complex physiological systems. The need for a more accurate exposure model is highlighted by the fact that NP behavior and subsequent bioresponses are highly dependent upon their surroundings. Therefore, standard in vitro models will likely produce inaccurate NP behavioral analyses and erroneous safety results. As such, the goal of this study was to develop an enhanced in vitro model for NP evaluation that retained the advantages of cell culture, but implemented the key physiological variables of accurate biological fluid and dynamic flow. In this study, a cellular microenvironment was modeled and created after an inhalation exposure scenario. This system comprised of A549 lung epithelial cells, artificial alveolar fluid (AAF), and biologically accurate dynamic flow...Continue Reading

References

Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Sep 2, 2003·Journal of Environmental Monitoring : JEM·Woodhall StopfordTom Brock
Oct 8, 2005·Particle and Fibre Toxicology·Günter OberdörsterUNKNOWN ILSI Research Foundation/Risk Science Institute Nanomaterial Toxicity Screening Working Group
Apr 13, 2006·Nano Letters·B Devika ChithraniWarren C W Chan
Nov 14, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Justin G TeeguardenJoel G Pounds
Dec 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Manish SethiMarc R Knecht
Nov 6, 2009·Physical Chemistry Chemical Physics : PCCP·Kenji KatayamaTakeshi Eitoku
Jun 11, 2010·Nano Letters·Rochelle R ArvizoPriyabrata Mukherjee
Jul 16, 2010·Journal of Nanobiotechnology·Selvaraj BarathmanikanthSangiliyandi Gurunathan
Dec 2, 2010·Particle and Fibre Toxicology·Paul M HinderliterJustin G Teeguarden
Mar 2, 2011·Environmental Health Perspectives·Rebecca Kessler
Apr 26, 2011·Nature Nanotechnology·Eun Chul ChoYounan Xia
Aug 3, 2011·Analytical and Bioanalytical Chemistry·Justin M ZookRobert I MacCuspie
Dec 7, 2011·Environmental Science & Technology·Rui MaGregory V Lowry
Dec 16, 2011·Chemical Society Reviews·Shahriar SharifiMorteza Mahmoudi
Dec 20, 2011·Toxicology and Applied Pharmacology·Sumit AroraKishore M Paknikar
Aug 3, 2012·Accounts of Chemical Research·Kim M TsoiWarren C W Chan
Aug 3, 2012·Annals of Biomedical Engineering·Jiro KusunoseKatherine W Ferrara
Aug 16, 2012·Accounts of Chemical Research·Motao ZhuYuliang Zhao
Nov 7, 2012·Journal of Biochemical and Molecular Toxicology·Ramakrishna Podila, Jared M Brown
Aug 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Madeleine C DeBrosseSaber M Hussain
Aug 21, 2013·ACS Applied Materials & Interfaces·Emily A UntenerSaber M Hussain
Nov 12, 2013·Archives of Pharmacal Research·Eun Hye JeongHyukjin Lee
Jan 17, 2014·Journal of Visualized Experiments : JoVE·Mark E KusekBryan P Hurley
Mar 22, 2014·International Journal of Molecular Sciences·Eleonore Fröhlich, Sharareh Salar-Behzadi
May 17, 2014·Environmental Pollution·Niksa OdzakLaura Sigg
Sep 28, 2014·Colloids and Surfaces. B, Biointerfaces·Kristen K ComfortSaber M Hussain
Dec 17, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Laura K Braydich-StolleSaber M Hussain
Feb 12, 2015·Chemical Society Reviews·W J StarkA Hafner

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Citations

Apr 30, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Nicholas J BraunKristen K Comfort
Jul 14, 2020·Journal of Biochemical and Molecular Toxicology·Cunjing ZhengLi Li
Sep 16, 2017·Applied Microbiology and Biotechnology·Joseph R ShileyJayne B Robinson
Dec 4, 2020·Emerging Topics in Life Sciences·Ainoa GuinartTeresa D Tetley
Aug 14, 2020·Bioconjugate Chemistry·Valentina FranciaDominik Witzigmann
May 18, 2021·Journal of Tissue Engineering·Roberta NossaArti Ahluwalia

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Methods Mentioned

BETA
imaging techniques
dynamic light scattering
fluorescence microscopy
light microscopy
fluorescence imaging
Assay

Software Mentioned

Graph Pad Prism
QCapture Pro Imaging

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