Abstract
Differentiation process of mesenchymal stem cells (MSCs) into adipocyte is involved in obesity. Multiple factors such as Ca2+ play important roles in different stages of this process. Because of the complicated roles of Ca2+ in adipogenesis, the aim of present investigation was to study the influx and efflux of Ca2+ into and out of the cells during adipogenesis. Adipose-derived MSCs were used to differentiate into adipocytes. MSCs were exposed to 2.5 mM Ca2+ or 1.8 mM Ca2+ plus calcium ionophore, A23187, for 3 days. Lipid staining, triglycerides (TG) content, and glyceraldehyde phosphate dehydrogenase (GAPDH) activity were evaluated to confirm the efficiency of the differentiation. Gene expression of GLUT4, PPARγ2, RAR-α, and calreticulin, as well as the protein levels of GLUT4 and PPARγ2 were determined. Ca2+ and in particular Ca2+ plus A23187 significantly lowered the efficiency of differentiation accompanied by decrease in intracellular TG deposits, GAPDH activity and alleviation of gene, and protein levels of GLUT4 and PPARγ2. While calreticulin and RAR-α were remarkably upregulated in A23187 group. This study showed the inhibitory effects of calcium in adipogenesis. Additionally, it indicated the greater inhibitory effect ...Continue Reading
References
Jun 1, 1996·Differentiation; Research in Biological Diversity·J M Ntambi, T Takova
Jan 1, 1996·The American Journal of Physiology·B H JonesN Moustaid
Dec 1, 1996·Endocrinology·C W MillerJ M Ntambi
Jun 8, 1999·The Journal of Biological Chemistry·R F Morrison, S R Farmer
Oct 4, 2000·Physiological Genomics·H ShiM B Zemel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 28, 2002·The Journal of Biological Chemistry·Joel W Neal, Neil A Clipstone
Jan 8, 2003·Blood·Sophie LaunayBéla Papp
Nov 9, 2004·Experimental Cell Research·Brian JensenKamil A Akanbi
Apr 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Julie R JonesMark A Magnuson
Mar 10, 2007·Circulation Research·Li Li ZhangMartin Tepel
Jun 15, 2007·Domestic Animal Endocrinology·M E FernyhoughM V Dodson
Jul 9, 2008·The Journal of Cell Biology·Jacopo Meldolesi
Jul 16, 2008·Molecular and Cellular Biochemistry·Mariana Cifuentes, Cecilia V Rojas
Apr 15, 2009·Molecular and Cellular Biology·Daniel C Berry, Noa Noy
Sep 18, 2010·DNA and Cell Biology·Obeid M MalekshahJavad Behravan
Nov 1, 2011·Molecular and Cellular Biochemistry·Yong-Han HeChang-Hao Sun
Mar 21, 2012·The American Journal of Pathology·Masanori HisaokaMitsuhiro Nakamoto
Dec 12, 2012·Biochimica Et Biophysica Acta·Tomáš MráčekJosef Houštěk
Feb 27, 2014·JAMA : the Journal of the American Medical Association·Cynthia L OgdenKatherine M Flegal
May 31, 2017·Immunopharmacology and Immunotoxicology·Majid BahabadiIraj Khodadadi
Aug 30, 2017·Archives of Physiology and Biochemistry·Farjam GoudarziAli Akbar Saboor-Yaraghi
Citations
Jun 6, 2018·Journal of Cellular Physiology·Farjam GoudarziIraj Khodadadi
Feb 21, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan Qin TanLai K Leung
Jun 15, 2019·International Journal of Molecular Sciences·Ziguo SongChao Sun
Jul 6, 2018·Genes·Yueyuan XuXinyun Li
Jun 27, 2019·International Journal of Molecular Sciences·Fenglin ZhangSongbo Wang
Dec 2, 2020·Animals : an Open Access Journal From MDPI·Jiahao ShaoSongjia Lai
Sep 10, 2020·Physiological Research·H WuC Shen
Jan 27, 2020·Molecular and Cellular Probes·Xingzhou QuLiancheng Shan
Mar 7, 2021·International Journal of Molecular Sciences·Krystyna Szymandera-BuszkaMaciej Jarzębski
Jun 27, 2019·Journal of Agricultural and Food Chemistry·Fenglin ZhangSongbo Wang
Aug 31, 2021·Clinical Therapeutics·Mojgan MorvaridzadehJavad Heshmati
Sep 24, 2021·Genetics, Selection, Evolution : GSE·Miguel Gozalo-MarcillaRoger Ros-Freixedes
Oct 15, 2021·Endocrinology and Metabolism·Julio C SánchezAndrés M García