The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo

Cancer Management and Research
Wenwen ZhangXueqiong Zhu

Abstract

The objective of the study was to explore the role of calgranulin B gene on the biological behavior of squamous cervical cancer. Differential transcription in calgranulin B gene between human papillomavirus (HPV)-positive and negative cervical cancer groups was identified, and the relationship between calgranulin B gene and matrix metalloproteinase (MMP) genes were explored using The Cancer Genome Atlas database. Subsequently, the role of calgranulin B on the cell proliferation, apoptosis, invasion and migration was investigated, through overexpression and/or underexpression of calgranulin B in cervical cancer cells. In addition, the effect of calgranulin B on the growth of the cervical cancer was studied via constructing xenograft model in BALB/c nude mice that either overexpressed or underexpressed calgranulin B. Calgranulin B gene transcription in cervical cancer was highly correlated with the high-risk HPV-16 and HPV-45. In addition, overexpression of calgranulin B increased cell proliferation, invasion and migration, whereas it did not significantly affect cell apoptosis. This effect was also confirmed by calgranulin B knockdown assay. Additionally, we found that the transcription of calgranulin B gene was negatively corre...Continue Reading

Methods Mentioned

BETA
electrophoresis
xenograft
enzyme-linked immunosorbent assay
ELISA
PCR
transfection
flow cytometry
xenografts

Software Mentioned

SPSS
Limma
Quantity
One
edgeR
R

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