The role of CCAAT/enhancer binding protein (C/EBP)-alpha in osteogenesis of C3H10T1/2 cells induced by BMP-2.

Journal of Cellular and Molecular Medicine
Qiming FanK Dai

Abstract

The balance between osteogenesis and adipogenesis of mesenchymal stem cells is disrupted in various human diseases. Investigating the mechanisms that fine-tune this balance is of medical importance. Identification of potential target gene which can be used to study the relationship between them could be really helpful for this purpose. In the current study, we used C3H10T1/2 as model cells and through which two models of both osteogenesis induced by bone-morphogenetic protein (BMP)-2 and transdifferentiation from osteogenesis to adipogenesis were established. We investigated the role of CCAAT/enhancer binding protein (C/EBP)-alpha in these two systems. Then from epigenetic point of view, we elucidated the underlying molecular mechanisms preliminarily. The results showed that down-regulations of both C/EBP-alpha expression and its inducibility in response to insulin, fetal bovine serum, methylisobutylxanthine and dexamethasone (IFMD) adipogenic hormonal cocktail were observed in terminal stage of osteogenesis of C3H10T1/2 cells induced by BMP-2. And overexpression of C/EBP-alpha could lead to inhibition of osteogenesis differentiation and rescue attenuation of potential of adipogenic conversion in this process. Furthermore, we p...Continue Reading

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Citations

Dec 24, 2010·Nucleic Acids Research·Andreas ProkeschZlatko Trajanoski
May 21, 2011·Cells, Tissues, Organs·Junsheng FengShuo Chen
Jun 17, 2015·Journal of Biochemistry·Yong-Xing ZhangQing-Hua Zhao
Jun 11, 2016·Cellular Signalling·Yu-Gang WangKe-Rong Dai
Apr 1, 2020·International Journal of Molecular Sciences·Shanmugam MuruganandanChristopher J Sinal
Jan 27, 2019·Journal of Orthopaedic Surgery and Research·Rui Ma, Dagang Guo
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jun-Jie GuanYa-Ping Guo
Nov 2, 2020·Journal of Materials Science. Materials in Medicine·Rui MaWei Wang
Apr 29, 2021·BMC Musculoskeletal Disorders·Wenting XuYoujia Xu

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