The role of CD14 and Toll-like receptor 4 of Kupffer cells in hepatic ischemia-reperfusion injury in rats

Transplantation Proceedings
X LuanM Li

Abstract

The objective of this study was to study the role of CD14 and Toll-like receptor 4 (TLR4) in Kupffer cells (KCs) on ischemia reperfusion injury (IRI) in rat liver grafts. Isolated KCs were obtained from control, IRI, and IRI plus anti-CD14 antibody groups. We measured messenger RNA (mRNA) and protein expression of the lipopolysaccharide receptor CD14 and TLR4, nuclear factor kappa B (NF-κβ) activity, and TNF-α levels. mRNA and protein expressions of CD14 and TLR4 were significantly higher in the IRI than in the control group, as were protein expressions of CD14 and TLR4 by flow cytometry and by Western blots. NF-κβ activity and tumor necrosis factor-α level in the IRI group were significantly higher than in the control group (3.17 ± 0.21 and 0.28 ± 0.03 vs 654.2 ± 3.6 pg/mL and 147.4 ± 1.1 pg/mL; t value = 4.11 and 4.29 for each; P < .01). Compared with the IRI group they were greatly decreased after anti-CD14 antibody treatment: 2.14 ± 0.17 vs 3.17 ± 0.21, 298.7 ± 1.8 pg/mL vs 654.2 ± 3.6 pg/mL (t value = 2.52 and 2.92 for each; P < .05). They were still significantly higher than the control group (t values of 3.01 and 3.27 for each; P < .01). IRI up-regulated CD14 and TLR4 gene expression in KCs, and subsequently activated NF...Continue Reading

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Citations

Sep 4, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Yan QinWeizhen Zhang
Mar 11, 2015·Clinical & Experimental Ophthalmology·Dana MorzaevNitza Goldenberg-Cohen
Jan 29, 2019·Bioscience, Biotechnology, and Biochemistry·Zuotian HuangZhongjun Wu
May 10, 2013·Shock·Changchun CaiTimothy R Billiar

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