The role of CD4 molecules in the induction phase of contact hypersensitivity cytokine profiles in the skin and lymph nodes

Immunology
H FujisawaD N Sauder

Abstract

We have previously demonstrated that CD4 gene-targeted mutant mice (CD4- mice) demonstrate hyporesposiveness in contact hypersensitivity (CHS) suggesting that CD4 molecules are required for optimal induction of CHS. In the present study, we wished to examine the mechanisms of this hyporesponsiveness, in particular, we examined whether cytokines were altered in the skin and lymph nodes of CD4- mice following exposure to the contact allergen dinitrofluorobenzene (DNFB). Cytokine mRNA in the ear skin and draining lymph nodes (DLN) were examined by reverse transcription-polymerase chain reaction (RT-PCR) at various times after sensitization. Skin cytokine patterns revealed that in normal mice, interleukin (IL)-2, interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha mRNA levels increased at 12 hr sensitization, whereas these cytokines were below the level of detection in CD4- mice. In the DLN of normal mice following the hapten application, sequential upregulation of cytokine mRNA including IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-10, IFN-gamma and TNF-alpha was found. No change was seen for IL-1 alpha, IL-1 beta, IL-10 and TNF-alpha and IL-2, IL-4 and IFN-gamma mRNA levels were below the level of detection in DLN from CD4- mi...Continue Reading

References

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Citations

Mar 2, 2005·The Journal of Investigative Dermatology·Pierre Saint-MezardFrédéric Bérard
Dec 14, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Anne Deen Christensen, Claus Haase
Jun 9, 2007·The American Journal of Pathology·Rei WatanabeKunihiko Tamaki
Jun 1, 2005·The American Journal of Cardiology·Anthony E Bolton
May 26, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Haruna NiwaHiroo Yokozeki
Dec 17, 2005·Immunity·Daniel H KaplanMark J Shlomchik
Jun 28, 2011·The American Journal of Pathology·Yoshihiro YamamotoTakahiro Satoh
Nov 11, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Donghong GaoDavid A Lawrence
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M S ExtonM Schedlowski
Sep 26, 2000·Journal of Cutaneous Medicine and Surgery·G M ShivjiD N Sauder

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