The role of cellular prion protein in lipid metabolism in the liver.

Prion
Amandeep Singh AroraInga Zerr

Abstract

Cellular prion protein (PrPC) is a plasma membrane glycophosphatidylinositol-anchored protein and it is involved in multiple functions, including neuroprotection and oxidative stress. So far, most of the PrPC functional research is done in neuronal tissue or cell lines; the role of PrPC in non-neuronal tissues such as liver is only poorly understood. To characterize the role of PrPC in the liver, a proteomics approach was applied in the liver tissue of PrPC knockout mice. The proteome analysis and biochemical validations showed an excessive fat accumulation in the liver of PrPC knockout mice with a change in mRNA expression of genes linked to lipid metabolism. In addition, the higher Bax to Bcl2 ratio, up-regulation of tgfb1 mRNA expression in PrPC knockout mice liver, further showed the evidences of metabolic disease. Over-expression of PrPC in fatty acid-treated AML12 hepatic cell line caused a reduction in excessive intracellular fat accumulation; shows association of PrPC levels and lipid metabolism. Therefore, based on observation of excessive fat globules in the liver of ageing PrPC knockout mice and the reduction of fat accumulation in AML12 cell line with PrPC over-expression, the role of PrPC in lipid metabolism is des...Continue Reading

References

Jun 1, 1992·Neurology·M TanakaT Yuasa
Oct 1, 1995·The Journal of General Virology·M HoriuchiM Shinagawa
Dec 10, 1998·The American Journal of Pathology·K IkedaK Kaneda
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Jun 6, 2000·Nature·S KerstenW Wahli
Jul 18, 2001·The Journal of General Virology·M MoudjouC La Bonnardière
Jan 13, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·M den BoerJ A Romijn
May 4, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Meagan J WalshElizabeth E Powell
Jun 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anna WieckowskaAriel E Feldstein
Apr 9, 2008·Physiological Reviews·Rafael LindenRicardo R Brentani
May 24, 2008·Protein Engineering, Design & Selection : PEDS·Tetsuya KadonosonoMitsuyoshi Ueda
Mar 12, 2010·Critical Reviews in Biochemistry and Molecular Biology·Maggie S Strable, James M Ntambi
Feb 25, 2011·The Journal of Nutrition·Mohamed A AbdelmegeedByoung-Joon Song
Aug 13, 2011·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Angela KuhlaBrigitte Vollmar
Mar 1, 2012·World Journal of Gastroenterology : WJG·Vincent BraunersreutherFabrizio Montecucco
Jun 8, 2012·Molecular Reproduction and Development·Oscar A PeraltaWillard H Eyestone
Jun 19, 2013·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Emine B YalcinRoberta S King
Jan 18, 2014·Cell Death & Disease·K Wang
Mar 13, 2014·Acta Pharmacologica Sinica·Qian-qian TuHong-yang Wang
May 20, 2014·International Journal of Molecular Sciences·Fatiha Nassir, Jamal A Ibdah
Jun 27, 2014·World Journal of Gastroenterology : WJG·Wilhelmus J KwantenSven M Francque
Oct 6, 2015·Trends in Pharmacological Sciences·Giuseppe Danilo NorataAlberico L Catapano
Dec 5, 2018·Molecular and Cellular Endocrinology·Jae-Kyo JeongSang-Youel Park

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Citations

May 30, 2021·NPJ Systems Biology and Applications·Yassene MohammedChristoph H Borchers

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Methods Mentioned

BETA
electrophoresis
PCR
light microscopy

Software Mentioned

GEne SeT AnaLysis Toolkit
Excalibur
IPA
Inguniety
Mascot
GraphPad
Prism
Raw2MSM
LightCycler 480
Delta2D

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