The role of cGMP in the relaxation to nitric oxide donors in airway smooth muscle

European Journal of Pharmacology
K Stuart-SmithKeith A Jones

Abstract

The aim of this study was to determine the effect of the soluble guanylyl cyclase inhibitors methylene blue and LY83583 (6-anilino-5,8-quinolinedione) on relaxation and increases in intracellular guanosine 3',5'-cyclic monophosphate (cGMP) concentration ([cGMP]i) induced by sodium nitroprusside, 3-morpholinosydnonimine (SIN-1) and diethylamine-nitric oxide (NO) in porcine tracheal smooth muscle in vitro. We measured (1) the effect of NO donors on isometric force and [cGMP]i and (2) the ability of methylene blue and LY83583 to antagonize these effects. In muscle strips contracted with carbachol (0.1-0.3 microM), both sodium nitroprusside and diethylamine-NO caused relaxation and an increase in [cGMP]i. By contrast, SIN-1 caused a relaxation which was not associated with a concomitant increase in [cGMP]i. Methylene blue (10 microM) and LY83583 (10 microM) completely blocked the increase in [cGMP]i induced by sodium nitroprusside and diethylamine-NO; however substantial relaxation remained. It is concluded that in porcine airway smooth muscle, (1) relaxation induced by some NO donors may occur without a concomitant increase in [cGMP]i; and (2) whereas relaxation induced by some NO donors may be associated with increases in [cGMP]i...Continue Reading

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Citations

Jul 27, 2000·Journal of Cellular Physiology·J A CarvajalC P Weiner
Mar 4, 1999·European Journal of Pharmacology·R K PrasadF Ismail-Beigi
Dec 20, 2011·Nitric Oxide : Biology and Chemistry·Elia TfouniDouglas Wagner Franco
Dec 24, 2003·Autonomic & Autacoid Pharmacology·C H JunU D Sohn
Jan 1, 2012·Canadian Journal of Chemistry·Moshood K MorakinyoReuben H Simoyi
Mar 11, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·C M PabelickK A Jones
Oct 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ahmed M HamadAlan J Knox
Sep 25, 2017·Physiological Research·M AntosovaA Bencova
Jun 3, 2021·Veterinary Sciences·Katrin RöhmJana Seifert

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