PMID: 6170611Sep 1, 1981Paper

The role of chromosomal proteins in the induction of a differential staining of sister chromatids by light

The Histochemical Journal
C H Buys, J Osinga

Abstract

Fixed chromosomes of human lymphocytes, cultured in the presence of bromodeoxyuridine (BrdU) during two cell cycles, were exposed to near-ultraviolet irradiation, stained with Giemsa, and after destaining, were subjected to either Coomassie Blue or Feulgen--Schiff staining. A differential reaction of sister chromatids was first revealed by Coomassie Blue staining. Differential staining with Giemsa required a longer irradiation time. This appeared to be reduced after the addition of dithiodipyridine to the cells during their last few hours of culture. The differential pattern obtained after Coomassie Blue staining was the inverse of that obtained after Giemsa staining. From these findings we concluded that the induction of sister chromatid differentiation by light in BrdU-substituted DNA containing chromosomes occurs primarily via chromosomal proteins, presumably by differential breakage of their disulphide bonds. The results of the Feulgen--Schiff staining indicated that differential depurination of BrdU-containing DNA could occur, although only after very prolonged irradiation. A faint though distinctly differential Feulgen--Schiff pattern of sister chromatid staining, resulting from differential removal of DNA, was observed a...Continue Reading

References

Sep 1, 1976·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·M S LinG N Donnell
Feb 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P T Kjellstrand
Jan 1, 1977·Annual Review of Genetics·S Wolff
May 16, 1978·Human Genetics·G Kosztolányi, E M Bühler
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S A Latt
Feb 1, 1974·Experimental Cell Research·A T Sumner
Apr 28, 1970·Biochimica Et Biophysica Acta·A Sadgopal, J Bonner
May 14, 1963·Biochimica Et Biophysica Acta·S Fazekas de St GrothA Datyner

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