The role of circulating food antigen-specific lymphocytes in food allergic children with atopic dermatitis

The British Journal of Dermatology
R ReekersT Werfel

Abstract

In this study we evaluated antigen-specific in vitro responses of peripheral blood lymphocytes to lipopolysaccharide (LPS)-depleted food allergens in children who reacted to food challenge (cow's milk or hen's egg) with a deterioration of their atopic dermatitis (AD). Some of the children showed immediate symptoms (urticaria, bronchial asthma or gastrointestinal symptoms) as well. The proliferation of casein-stimulated lymphocytes from children reacting to cow's milk (age 0.7-5.9 years) was significantly higher (P < 0.01) than the proliferation of lymphocytes from 15 children with AD without milk allergy (age: 2.1-9.1 years). Twenty-eight T-cell clones (TCC) were established from the blood of three children sensitized to cow's milk and hen's egg who reacted to double-blind, placebo-controlled oral food challenge both with a deterioration of AD and with immediate symptoms. Surprisingly, 16 of 28 casein- or ovalbumin-specific TCC were CD8+. All TCC produced high amounts of IFN-gamma upon stimulation with concanavalin A. In addition, 75% of the CD4+ TCC and 44% of the CD8+ TCC secreted IL-4. Our results indicate that: (i) food-specific proliferation of blood lymphocytes can be detected in patients with clinically relevant food all...Continue Reading

References

Nov 1, 1992·The Journal of Pediatrics·A W Burks, H A Sampson
Aug 6, 1992·The New England Journal of Medicine·H A SampsonJ P Rosen
Mar 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L RäsänenT Reunala
Apr 1, 1991·Journal of the American Academy of Dermatology·A KappE Schöpf
Aug 1, 1990·The Journal of Allergy and Clinical Immunology·N KondoT Orii
Dec 1, 1988·International Journal of Bio-medical Computing·L W StrijboschW A Buurman
Sep 1, 1988·The Journal of Pediatrics·A W BurksM A Shirrell
Dec 24, 1987·Journal of Immunological Methods·T E KarplusC B Wilson
Aug 1, 1986·The Journal of Pediatrics·D J HillC S Hosking
Nov 1, 1985·The Journal of Pediatrics·H A Sampson, C C McCaskill
Jan 1, 1980·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·T AmemiyaM Uehara
Feb 1, 1995·The Journal of Clinical Investigation·K J Abernathy-CarverD Y Leung
Feb 1, 1994·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·B NiggemannH A Sampson

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Citations

Apr 25, 2012·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·K WichmannT Werfel
May 27, 2011·Current Allergy and Asthma Reports·Annice HeratizadehThomas Werfel
Jun 5, 2003·Clinics in Dermatology·Hugh A Sampson
Jan 23, 1999·Current Opinion in Immunology·T T MacDonald
May 9, 2012·Nature Medicine·Sabina A Islam, Andrew D Luster
Jul 25, 1998·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·D E CampbellA S Kemp
Jun 18, 2011·Journal of Paediatrics and Child Health·Dianne E Campbell
Sep 12, 1998·Lancet·D Rudikoff, M Lebwohl
Feb 5, 1999·Clinical and Experimental Immunology·A LonatiG De Panfilis
Jan 22, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M LehtoH Alenius
Jul 31, 2010·Immunology and Allergy Clinics of North America·Jean-Christoph Caubet, Philippe A Eigenmann
Jun 29, 2010·Seminars in Cutaneous Medicine and Surgery·Ki-Young Suh
Aug 19, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Laurie A Lee
May 18, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K BreuerT Werfel
Jun 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R P SchadeE van Hoffen
Dec 21, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I TsugeA Urisu
Feb 9, 1999·The Journal of Allergy and Clinical Immunology·M OhsakiS Chiba
May 20, 1998·Dermatologic Clinics·R Stadler
Sep 17, 2014·Clinical and Experimental Dermatology·Y-S YangC-W Park
Jun 30, 2015·The Journal of Pediatrics·Tuyet Ann NguyenLawrence F Eichenfield
Feb 15, 2015·Gastroenterology·Rudolf ValentaSandra Pahr
Jan 30, 2010·The Journal of Allergy and Clinical Immunology·Kathleen C Barnes
May 30, 2009·Advances in Immunology·Michiko K OyoshiRaif S Geha
Jan 8, 2005·The Journal of Allergy and Clinical Immunology·Maritta PykäläinenRiitta Lahesmaa
Apr 12, 2001·Allergy·T Werfel
Jan 15, 2018·Clinical Reviews in Allergy & Immunology·Benjamin P Davis
Oct 28, 2004·Molecular Nutrition & Food Research·Barbara Bohle
Sep 7, 2004·Current Opinion in Allergy and Clinical Immunology·Thomas Werfel, Kristine Breuer

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