PMID: 6976918Jan 1, 1981Paper

The role of complement in the function of the monocyte - macrophage system

Haematology and Blood Transfusion
H U Schorlemmer

Abstract

In parallel to the behavior of many agents that activate complement via the alternative pathway and can stimulate macrophages to secrete lysosomal enzymes we investigated the interaction of mouse peritoneal macrophages cultured in a serum-free medium with various stimuli such as zymosan, polyanions, collagen type II, and immune complexes prepared from tetanus toxoid and pooled human anti-tetanus toxoid F(ab)2. All these stimuli induced the release of hydrolytic enzymes from macrophage in culture. The release was time and dose dependent and is not associated with loss of the cytoplasmic enzyme lactate dehydrogenase or any other sign of cell death. The mechanism of macrophage activation by these various agents is unknown. Macrophages have surface receptors for Fc and C3b with the capacity to bind immune complexes or C3b, respectively, and this is followed by activation of the cells. Activation via the Fc part can be excluded in these experiments. The possibility therefore arose that macrophages might be stimulated by endogenous C3 via the C3b receptor, since it is known that all the substances mentioned above can activate C3. To confirm this hypothesis we tried to inhibit this reaction by using an anti-C3-Fab preparation. There w...Continue Reading

Citations

Oct 1, 1988·Immunology and Cell Biology·P D Cooper, E J Steele

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.