The role of Cryptosporidium parvum-derived phospholipase in intestinal epithelial cell invasion

Parasitology Research
Richard C G PollokMichael J G Farthing

Abstract

In the Cryptosporidium parvum-infected intestinal epithelial cell, the parasite occupies an unusual extracytoplasmic location at the luminal surface, but how the invading zoites interact with the host cell to achieve this niche is poorly understood. This study examined the role of secretory phospholipase A(2) (sPLA(2)), a known virulence factor for several pathogenic microorganisms, in establishing C. parvum intracellularly. Initially, it was established that there was sPLA(2) activity in homogenates of C. parvum oocysts. C. parvum reproduction in two human enterocyte cell lines was significantly reduced by a specific PLA inhibitor, p-bromophenacylbromide, and by sheep anti-sPLA(2) antibodies developed against PLA(2) of bee ( Apis mellifera) venom. Treatment of either C. parvum sporozoites or enterocytes with sPLA(2) derived from cobra ( Naja naja) venom before initiation of infection increased the numbers of intracellular parasites. Thus, C. parvum PLA(2 )may play an important part in establishing the parasite within the enterocyte.

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Citations

Jan 9, 2008·Parasitology Research·Luiz Felipe Domingues PasseroMarcos H Toyama
Jul 3, 2013·Microbes and Infection·Abdur RubYusuf Akhter
Jun 27, 2009·Parasitology Research·M ShahiduzzamanA Daugschies
Sep 6, 2011·International Journal for Parasitology·P Karanis, H M Aldeyarbi
Apr 5, 2011·Microbes and Infection·Steven P O'Hara, Xian-Ming Chen
Mar 1, 2005·Trends in Parasitology·Huw V SmithAnthony M Grimason
May 5, 2004·Best Practice & Research. Clinical Gastroenterology·David W K Acheson, Stefano Luccioli
Sep 20, 2008·Trends in Parasitology·Hanna BorowskiR C Andrew Thompson
Jan 1, 2013·Molecular and Biochemical Parasitology·María Laura BelaunzaránElvira Luisa Durante de Isola
May 24, 2011·Enzyme Research·María Laura BelaunzaránElvira Luisa Durante de Isola
May 22, 2010·Chemistry & Biodiversity·Niti VaneeGregory A Buck
Jan 9, 2013·Clinical Microbiology Reviews·Maha BouzidKevin M Tyler
Mar 22, 2006·Cellular Microbiology·Dominique Buzoni-GatelLloyd H Kasper
Apr 1, 2014·Parasitology·Matthias Lendner, Arwid Daugschies
Sep 13, 2003·Parasite Immunology·Michael J G Farthing
Dec 14, 2004·The Journal of Biological Chemistry·Purnima BhanotVictor Nussenzweig

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