PMID: 11345144May 10, 2001Paper

The role of cyclic AMP in the pathogenesis of glucose desensitization in rat pancreatic islets

Pancreas
H YoshikawaH Nawata

Abstract

Adenosine-3',5'-cyclic monophosphate (cyclic AMP) promotes exocytosis of insulin in pancreatic beta cells. This study was performed to investigate the role of cyclic AMP in the pathogenesis of glucose desensitization in rat pancreatic islets. In islets cultured with high glucose for 48 hours, 27 mmol/L glucose-induced insulin release was markedly impaired, while 3.3 mmol/L glucose-or arginine-induced insulin release was enhanced, indicating glucose desensitization. Islet cyclic AMP content was 190% enhanced in high glucose-culture islets for 48 hours. In islets cultured with dibutyryl-cyclic AMP (dbcAMP) or 3-isobutyl methy-xanthine (IBMX), islet insulin content or 27 mmol/L glucose-induced insulin release was deteriorated. In contrast, 3.3 mmol/L glucose- or arginine-induced insulin release was increased, which was similar to glucose-desensitized islets. Wash-out of dbc AMP for the last 24 hours of the 48-hour culture period restored impaired high glucose-induced insulin release in the same manner as wash-out of high glucose. Diazoxide, the KATP channel opener, also restored impaired high glucose-induced insulin release from dbcAMP-cultured islets. The data suggest that enhancement of cyclic AMP in high glucose-culture islets ...Continue Reading

References

Sep 1, 1976·The Journal of Clinical Investigation·J P PalmerJ W Ensinck
Jan 1, 1986·Diabetes/metabolism Reviews·G C WeirS Bonner-Weir
Nov 1, 1986·Pflügers Archiv : European journal of physiology·G TrubeT Ohno-Shosaku
Apr 1, 1973·The Journal of Clinical Investigation·R P Robertson, D Porte
Jul 1, 1972·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·T DeckertM Deckert
Oct 1, 1983·Molecular and Cellular Endocrinology·S G Laychock
Aug 30, 1995·Molecular and Cellular Endocrinology·S G Laychock

❮ Previous
Next ❯

Citations

May 22, 2010·Journal of Clinical Biochemistry and Nutrition·Julio C FraulobCarlos A Mandarim-de-Lacerda
Jul 7, 2009·Medical Hypotheses·Mark F McCartyFrancisco Contreras
Nov 10, 2004·Medical Hypotheses·Mark F McCarty
Jul 31, 2012·The Journal of Nutritional Biochemistry·M L Lazo de la Vega-MonroyC Fernandez-Mejia

❮ Previous
Next ❯

Related Concepts

Related Feeds