The role of cyclic AMP response element-binding protein in testosterone-induced differentiation of granular convoluted tubule cells in the rat submandibular gland

Archives of Oral Biology
J KimS Iseki

Abstract

The postnatal development of granular convoluted tubules (GCT) in the duct system of the rodent submandibular gland is known to be androgen-dependent, but the underlying molecular mechanism is unclear. To test the possible role of the transcription factor, cyclic AMP response element-binding protein (CREB), in the androgen-induced differentiation of GCT, the effect of testosterone on the expression and localization of epidermal growth factor (EGF), a marker of GCT cells, and of CREB was examined in the submandibular glands of immature 3-week-old rats. Northern blotting demonstrated increases in both EGF and CREB mRNA 1-4 days after testosterone administration. Immunoprecipitation also indicated that CREB protein was increased in amount with testosterone administration, and that induced CREB was phosphorylated at the serine residue as in the active form of CREB. In situ hybridization demonstrated that cells with CREB mRNA signal first appeared in the distal portions of striated ducts at 1 day and had increased in number by 4 days after giving testosterone, when cells with EGF mRNA signal became evident in the same duct portions. Immunohistochemistry also showed the occurrence of CREB protein in the nuclei of duct epithelial cell...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 1, 1975·The Anatomical Record·R Srinivasan, W W Chang
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D M Ignar-TrowbridgeK S Korach
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Aug 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E W Gresik
Aug 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T Barka
Apr 28, 1995·Molecular and Cellular Endocrinology·P E de RuiterA O Brinkmann
Jan 1, 1994·Microscopy Research and Technique·E W Gresik
Oct 1, 1996·Endocrinology·P ReinikainenO A Jänne
Jan 1, 1995·Critical Reviews in Eukaryotic Gene Expression·C ChangA Mizokami
Jul 1, 1996·The Histochemical Journal·Y H ZhuangP Tuohimaa
Jan 1, 1997·Annual Review of Biochemistry·M Montminy
May 6, 1999·The EMBO Journal·G CastoriaF Auricchio
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·A O BrinkmannJ Trapman
Jun 29, 2000·Annual Review of Biochemistry·A J Shaywitz, M E Greenberg

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Citations

Oct 20, 2009·Histochemistry and Cell Biology·Sunisa KeattikunpairojShoichi Iseki
Jan 28, 2012·Acta Histochemica Et Cytochemica·Ikuko AmanoYoshihiko Sawa
Mar 8, 2008·The Tohoku Journal of Experimental Medicine·Wiphawi HipkaeoShoichi Iseki
Mar 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wiphawi HipkaeoShoichi Iseki
Sep 2, 2006·Experimental Biology and Medicine·Juan Pablo PrestifilippoJuan Carlos Elverdin
Sep 1, 2004·Cellular Signalling·Mona JohannessenUgo Moens
Sep 5, 2009·Brain Research·Thuy-Vi V NguyenChristian J Pike

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