PMID: 36150Apr 12, 1979

The role of cysteine residues in the catalytic activity of glycerol-3-phosphate dehydrogenase

Biochimica Et Biophysica Acta
R E Smith, R MacQuarrie


Glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ 2-oxido-reductase, EC has been shown to be sensitive to inhibition by iodoacetate. The reaction of the enzyme with iodoacetate, which appears to be a simple bimolecular process, is accompanied by a corresponding loss of enzyme activity. In addition to changes in activity, the alkylation reaction was monitored by the incorporation of radioactivity from iodo[2-14C]acetate, by changes in amino acid composition, and by changes in the content of free sulfhydryl groups. It is concluded that there are two cysteine residues in the native dimeric enzyme which are essential for enzymic activity. The rate of inactivation was relatively insensitive to the presence of various compounds with the exception of NADH which markedly inhibited the reaction. Kinetic and binding studies showed that the binding of NADH prevents alkylation and, conversely, alkylation prevents NADH binding. From the pH dependence of the alkylation reaction, the pKa of the essential sulfhydryl groups was calculated to be 8.5 and it is suggested that the binding of coenzyme is independent of the state of ionization of these groups.


Jun 1, 1995·The International Journal of Biochemistry & Cell Biology·T C KoekemoerW Oelofsen
Jan 24, 2014·Redox Biology·Ryan J MaillouxWilliam G Willmore
Dec 19, 2014·Molecular BioSystems·D W Bak, E Weerapana
Feb 6, 2014·Physical Chemistry Chemical Physics : PCCP·Srabanti GhoshSomobrata Acharya


Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M J AdamsS S Taylor
May 1, 1970·Archives of Biochemistry and Biophysics·B M AndersonC N Wang
Feb 15, 1973·Journal of Molecular Biology·S A Bernhard, R A MacQuarrie
May 15, 1973·Journal of Molecular Biology·S T Rao, M G Rossmann
Aug 1, 1971·Archives of Biochemistry and Biophysics·T P FondyD B Rutherford
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jul 10, 1963·Biochemical and Biophysical Research Communications·T K LI, B L VALLEE

Related Concepts

Nadh Binding
Glycerol-3-Phosphate Dehydrogenase
Phosphate Measurement
Sulfhydryl Compounds
Chinchilla Rabbits
Enzyme Activity
Blood Enzyme Activity (Lab Test)

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.