The role of dsbA in colonization of the wheat rhizosphere by Pseudomonas fluorescens Q8r1-96

Microbiology
Olga MavrodiLinda S Thomashow

Abstract

Certain well-conserved genes in fluorescent Pseudomonas spp. are involved in pathogenic interactions between the bacteria and evolutionarily diverse hosts including plants, insects and vertebrate animals. One such gene, dsbA, encodes a periplasmic disulfide-bond-forming enzyme implicated in the biogenesis of exported proteins and cell surface structures. This study focused on the role of dsbA in Pseudomonas fluorescens Q8r1-96, a biological control strain that produces the antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) and is known for its exceptional ability to colonize the roots of wheat and pea. The deduced DsbA protein from Q8r1-96 is similar to other predicted thiol : disulfide interchange proteins and contains a conserved DsbA catalytic site, a pattern associated with the thioredoxin family active site, and a signal peptide and cleavage site. A dsbA mutant of Q8r1-96 exhibited decreased motility and fluorescence, and altered colony morphology; however, it produced more 2,4-DAPG and total phloroglucinol-related compounds and was more inhibitory in vitro to the fungal root pathogen Gaeumannomyces graminis var. tritici than was the parental strain. When introduced separately into a natural soil, Q8r1-96 and the dsbA mutant...Continue Reading

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Citations

Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Carmen M HerreraSusanne B von Bodman
Aug 29, 2006·Applied and Environmental Microbiology·Olga V MavrodiLinda S Thomashow
Oct 26, 2010·Journal of Bacteriology·Dmitri V MavrodiLinda S Thomashow
May 23, 2014·Journal of Proteomics·Vanessa Rodrigues PegosAndrea Balan
Jun 1, 2010·Environmental Microbiology Reports·Simon H MillerFergal O'Gara

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