The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia Linn. (Noni)

Evidence-based Complementary and Alternative Medicine : ECAM
Yougen WuGhee T Tan

Abstract

We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. The foremost goal of this study is to explore the role of endophyte-derived secondary metabolites in the purported anticancer properties of noni. To that end, culturable endophytic fungi resident within the healthy leaves and fruit of the plant were isolated and identified by molecular sequence analysis of the 5.8S gene and internal transcribed spacers (ITS). Purified organisms were subjected to in vitro fermentation in malt extract broth for 8 weeks under anaerobic conditions at room temperature (25°C), in order to simulate the conditions under which traditional fermented noni juice is prepared. The cytotoxic potential of organic extracts derived from the fermented broths of individual endophytes was then tested against three major cancers that afflict humans. Twelve distinct endophytic fungal species were obtained from the leaves and 3 from the fruit. Three of the leaf endophytes inhibited the growth of human carcinoma cell lines LU-1 (lung), PC-3 (prostate), and MCF-7 (breast) with IC50 values of...Continue Reading

References

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Citations

May 20, 2017·Phytotherapy Research : PTR·Mylena Andréa Oliveira TorresAna Lucia Abreu-Silva
Feb 23, 2019·International Journal of Environmental Research and Public Health·Nkemdinma Uche-OkereaforVuyo Mavumengwana
Aug 14, 2020·Journal of Fungi·Jacqueline Santos CruzLidilhone Hamerski
Aug 2, 2017·Asian Pacific Journal of Tropical Medicine· MinarniAkhmad Endang Zainal Hasan
Dec 8, 2020·Frontiers in Microbiology·Md Sohanur RahamanMd Amirul Islam

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Methods Mentioned

BETA
PCR
electrophoresis
genotyping
Assay

Software Mentioned

BLAST
WorkOut
Basic Local Alignment and Search Tool ( BLASTn )

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