The role of endoplasmic reticulum stress in the early stage of diabetic retinopathy.

Acta Diabetologica
Bin LiMin Hong Zhao

Abstract

The aim of this study was to evaluate the role of endoplasmic reticulum (ER) stress in diabetic retinopathy (DR) using in vitro and in vivo models. For the in vivo studies, diabetes was induced in rats, and retinal expression of glucose-regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), C/EBP homologous protein (CHOP), and vascular endothelial growth factor (VEGF) in groups of rats at 1, 3, and 6 months was assessed. For the in vitro studies, human retinal capillary endothelial cells (HRCECs) were cultured in the presence of varying glucose concentrations, and the expression of mRNA and protein for GRP78, ATF4, CHOP, and VEGF was assessed. The chosen glucose concentrations were reflective of those apparent in diabetic patients. Expression of VEGF and CHOP mRNA levels were significantly increased in diabetic rats compared to control rats at 1, 3, and 6 months (P < 0.05). In HRCECs cultured in the presence of high as well as variable glucose concentrations, CHOP expression and apoptosis were significantly increased (P < 0.05). However, GRP78 and ATF4 expression levels were unchanged. Our findings suggest that early progression of DR may be mediated by ER stress, but probably does not involve changes in ATF4 o...Continue Reading

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Citations

Nov 26, 2010·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Jun MakitaPeter F Kador
Dec 29, 2011·Experimental Diabetes Research·Wei-Kun HuBin Li
Sep 23, 2014·Cell Calcium·Agustin Guerrero-HernándezSilvia DeJesus
Dec 23, 2014·Journal of Diabetes Research·Jacey Hongjie MaSarah X Zhang
Aug 5, 2011·Journal of the Science of Food and Agriculture·Rong Fu ChenRen Fang Shen
Apr 13, 2021·Frontiers in Medicine·Qiaoyun GongXun Xu
Jun 30, 2021·Molecular Medicine Reports·Viviane Aline Oliveira SilvaFernando Luiz De Lucca

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