The role of epidermal growth factor receptor (EGFR) in male reproductive tract differentiation: stimulation of EGFR expression and inhibition of Wolffian duct differentiation with anti-EGFR antibody

Endocrinology
C Gupta

Abstract

We have shown previously that epidermal growth factor (EGF) plays a role in testosterone-dependent fetal Wolffian duct differentiation. To assess the role of EGF further, we examined EGF receptor (EGFR) expression during Wolffian duct differentiation and determined whether inhibition of EGFR results in inhibition of Wolffian duct differentiation. Using a polyclonal anti-EGFR antibody in the Western blot analysis, we have detected an immunoreactive band at the expected 180-kDa size, the molecular size for the EGFR protein, in the 18-day fetal male reproductive tract. The mouse 3T3 cell line, used as a positive control, also produced an immunospecific band at the same region. The intensity of the immunoreactive 180-kDa band in the 18-day male reproductive tract significantly decreased after pretreatment of the antibody with the 3T3 cell extract. Nonimmune serum produced no band at the 180- kDa region, suggesting EGFR specificity of the 180-kDa band. The level of the 180-kDa EGFR protein was found to be higher in the 18-day male than in the female reproductive tract. Testosterone increased expression of the EGFR level in the 18-day masculinized female reproductive tract, and it induced Wolffian duct morphogenesis. Under in vitro c...Continue Reading

Citations

Dec 12, 2001·Molecular and Cellular Endocrinology·I A HughesJ R Hawkins
May 31, 2014·Biochimica Et Biophysica Acta·Aki MurashimaGen Yamada
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