The role of estrogen receptor alpha in mediating chemoresistance in breast cancer cells.

Journal of Experimental & Clinical Cancer Research : CR
Zhinong JiangLinbo Wang

Abstract

Previous studies suggested that estrogen receptor alpha (ERα) plays an important role in the chemoresistance of breast cancers. However, large random trials failed to demonstrate any benefit of the concurrent estrogen antagonist tamoxifen on the chemotherapy efficacy. Thus, in the present study, the importance of the role of ERα in the chemoresistance of breast cancer cells was investigated. The ERα-transfected Bcap37 cells and natural ERα-positive T47D breast cancer cells were treated using chemotherapeutic agents with or without 17-beta estradiol (E2) pretreatment. Their viabilities were assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assays. The dead cell rates were determined using propidium iodide dye exclusion tests, and the expression levels of Bcl-2 and Bax were detected through Western blot analysis. The effects of E2 on the growth of breast cancer cells were also determined via cell growth curve and cell cycle analysis. ERα activation by E2 increased the sensitivity of natural ERα-positive T47D breast cancer cells to chemotherapeutic agents. However, the increase in ERα expression in ERα-negative Bcap37 breast cancer cells also significantly increased their resistance. These phenomena canno...Continue Reading

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Citations

Apr 17, 2015·BioMed Research International·Sabrina BimonteFrancesco Izzo
Aug 26, 2014·Journal of Experimental & Clinical Cancer Research : CR·Ting-Yu DaiChun-Meng Shi
Apr 20, 2013·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Eunjeong ChoiGunhee Kim
Jul 27, 2014·Experimental and Molecular Pathology·Haidan JinShuangping Liu
Jan 29, 2019·Journal of Molecular Cell Biology·Sue HauptYgal Haupt
Sep 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Chao-Yang XuLi-Ming Huang
Nov 4, 2017·Journal of Experimental & Clinical Cancer Research : CR·Jing TianJian Chen
Sep 5, 2020·Molecular Diversity·Nitish KumarPreet Mohinder Singh Bedi
Aug 3, 2019·Journal of Experimental & Clinical Cancer Research : CR·Francesca CirilloMarcello Maggiolini
Dec 3, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Hamed MontazeriMohammad Hossein Ghahremani
Jul 6, 2020·Environmental Science and Pollution Research International·Layasadat Khorsandi, Maryam Farasat
Jun 4, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Kumaraswamy Naidu Chitrala, Suneetha Yeguvapalli

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Methods Mentioned

BETA
flow cytometry
transfection
protein assay

Software Mentioned

SPSS

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