PMID: 6172468Jan 1, 1982Paper

The role of exogenous electron carriers in NAD(P)-dependent dehydrogenase cytochemistry studied in vitro and with a model system of polyacrylamide films

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
C J Van Noorden, J Tas

Abstract

The applicability of phenazine methosulfate, 1-methoxyphenazine methosulfate, menadione, and meldola blue as exogenous electron carriers for the cytochemical staining of nicotinamide adenine dinucleotide (phosphate) (NAD(P))-dependent dehydrogenases has been studied quantitatively with tetranitro BT in vitro and with a model system of polyacrylamide films incorporating either purified glucose-6-phosphate dehydrogenase or intact rat liver parenchymal cells. It was found that every assay in which a tetrazolium salt is used, whether or not an electron carrier is present, has to be carried out in darkness. Menadione did not appear to be useful, because electrons were not found to be transferred directly from reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) to this compound. Phenazine methosulfate at higher concentrations and meldola blue at concentrations optimal for carrying electrons to tetrazolium salts yielded a high level of "nothing dehydrogenase" activity in cell-containing films, but no inhibition of enzymatic activity was found. Factors involved in the interference of oxygen with tetrazolium salt reduction are discussed. 1-Methoxyphenazine methosulfate did not stain cellular compounds and caused only a very ...Continue Reading

Citations

Jul 20, 2000·Archives of Biochemistry and Biophysics·T Bernas, J W Dobrucki
Aug 1, 1984·The Histochemical Journal·R G Butcher, A W Evans
Sep 1, 1989·The Histochemical Journal·W M FrederiksG L Myagkaya
Nov 6, 1986·Journal of Immunological Methods·W D GeogheganR E Jordon
May 23, 1998·Acta histochemica·K ChikamoriK Sato
Sep 10, 1999·Journal of Microbiological Methods·V K BhupathirajuL Alvarez-Cohen
Jun 1, 1996·The Histochemical Journal·R J Van den Munckhof
Sep 1, 1982·The Histochemical Journal·C J Van Noorden, J Tas
Jun 1, 1983·The Histochemical Journal·C J Van NoordenI M Vogels
Oct 1, 1988·The Histochemical Journal·C J Van Noorden
Mar 1, 1983·The Histochemical Journal·C J Van Noorden
Jul 1, 1986·The Histochemical Journal·C J Van Noorden, R G Butcher
Jan 1, 1984·Progress in Histochemistry and Cytochemistry·C J Van Noorden
Dec 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Yasuo NakashimaYuichiro Nakaoki
Oct 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W M FrederiksC J van Noorden
Jun 20, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Severin, E Seidler
Dec 17, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R L Gardner
Jan 1, 1986·Histochemistry·M van der Ploeg, W A Duijndam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.