The role of extracellular signal-regulated kinases in the neuroprotection of limb ischemic preconditioning

Neuroscience Research
Rui-Li JinJie Qi

Abstract

To clarify the role of phosphorylated extracellular signal-regulated kinases (pERK1/2) in the neuroprotection of limb ischemic preconditioning (LIP) in rats, we investigated the expression of pERK1/2 using Western blot and flow cytometry in the hippocampus after LIP and the effect of pERK1/2 inhibitor PD 98059 on the neuroprotection of LIP against delayed neuronal death (DND) in the CA1 hippocampus normally induced by severe ischemic insult. It demonstrated that pERK1/2 in the hippocampus increased after LIP. In the CA1 hippocampus, ERK1/2 activation began to increase at 6h and reached peak at 12h after LIP, and decreased to sham level at 5d after LIP. On the other hand, in the CA3/DG, pERK1/2 enhanced at 1d, reached peak at 3d, and lasted to 5d after LIP. Pretreatment with PD 98059 before LIP blocked the neuroprotection of LIP in a dose-dependent manner. These findings supported that the upregulation of pERK1/2 in the CA1 hippocampus contributed to the neuroprotection of LIP against DND normally caused by the brain ischemic insult.

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Citations

Nov 14, 2013·Translational Stroke Research·Josef Anrather, John M Hallenbeck
Mar 19, 2011·Stroke; a Journal of Cerebral Circulation·Sebastian KochMiguel Perez-Pinzon
Nov 1, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Tao XuXiao-ming Deng
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Jul 27, 2014·Asian Pacific Journal of Tropical Medicine·Na ZhangFeng-Jiang Zhang
Jan 15, 2008·Journal of Neuroscience Research·Nik SaweHeng Zhao
Mar 14, 2019·The American Journal of Drug and Alcohol Abuse·Yosef Sarne
Jun 7, 2007·Neurochemical Research·Hong-Gang ZhaoQing-Jun Li
Jan 19, 2021·The Journal of Pharmacy and Pharmacology·Heena KhanThakur Gurjeet Singh

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