The role of Fe2+-induced lipid peroxidation in the initiation of the mitochondrial permeability transition

Archives of Biochemistry and Biophysics
Vladimir GogvadzeBruce N Ames

Abstract

Iron and iron complexes stimulate lipid peroxidation and formation of malondialdehyde (MDA). We have studied the effects of Fe2+ and ascorbate on mitochondrial permeability transition induced by phosphate and Ca2+. Iron is necessary for detectable MDA formation, but only Ca2+ and phosphate are necessary for the induction of membrane potential loss (Deltapsi) and Ca2+ release. Keeping the iron at a constant concentration and varying the Ca2+ level changed the mitochondrial Ca2+ retention times, but not the amount of MDA formation. The antioxidant butylated hydroxytoluene at low concentrations prevented MDA formation, but not mitochondrial Ca2+ release. Preincubation of mitochondria with Fe2+ decreased Ca2+ retention time in a concentration-dependent manner and facilitated Ca2+-stimulated MDA accumulation. Thus, Ca2+ phosphate-induced mitochondrial permeability transition (MPT) can be separated mechanistically from MDA accumulation. Lipid peroxidation products do not appear to participate in the initial phase of the permeability transition, but sensitize mitochondria toward MPT.

References

Jan 1, 1988·Free Radical Biology & Medicine·C Richter, B Frei
Jan 1, 1986·Journal of Free Radicals in Biology & Medicine·B R BaconC H Park
Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Apr 6, 1995·Biochemical and Biophysical Research Communications·D CeccarelliA Masini
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M K ShigenagaB N Ames
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·R Luft
Sep 24, 1993·Annals of the New York Academy of Sciences·J S Richardson
Oct 1, 1993·Gastroenterology·B R BaconR S Britton
Feb 1, 1997·Bioscience Reports·A E VercesiR F Castilho
Nov 21, 1997·Biochemical and Biophysical Research Communications·H Rottenberg, S Wu
May 15, 2001·Biological Signals and Receptors·M M Sugrue, W G Tatton
Feb 14, 2002·Archives of Biochemistry and Biophysics·Hani AtamnaBruce N Ames
Feb 8, 2002·Archives of Biochemistry and Biophysics·Vladimir GogvadzeBruce N Ames

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Citations

Mar 28, 2008·Journal of Bioenergetics and Biomembranes·Carlos A O SigoloMarisa H G Medeiros
Oct 2, 2007·Cell Biochemistry and Biophysics·Noemí GarcíaEdmundo Chávez
Feb 5, 2009·Neurochemical Research·Jéssie Haigert SudatiJoão Batista Teixeira Rocha
Feb 15, 2013·Chemical Biology & Drug Design·Rosivaldo S BorgesAlbérico B F da Silva
May 18, 2004·Brain Research·Nádia Aléssio Velloso BelléJoão Batista Teixeira Rocha
Oct 10, 2008·Aging Cell·Arnold Y SeoChristiaan Leeuwenburgh
Nov 18, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mohammad Jodeiri Farshbaf, Kamran Ghaedi
Mar 20, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Bibiana M GayAlcindo A Dos Santos

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