The role of flow cytometric immunophenotyping in improving the diagnostic accuracy in referred fine-needle aspiration specimens

Diagnostic Cytopathology
Eva SigstadBjörn Risberg

Abstract

Flow cytometric (FCM) immunophenotyping has an important role in the diagnostic work up of fine-needle aspiration (FNA) specimens obtained from lymphoid lesions. The objective of the present study was to evaluate the feasibility of this method with respect to referred FNA specimens. One hundred and two FNA specimens referred to our laboratory for FCM analysis during the last 3 years were studied. Specimens were sent, accompanied by cytological smears, from 11 distant hospitals by ordinary mail. The evaluation of potential B-cell monoclonality, the main diagnostic issue to be resolved using FCM, was possible in 86 of these 102 cases. The remaining 16 samples could not be analyzed or adequately interpreted because of sparse or necrotic material. A monoclonal B-cell population was found in 17/86 satisfactory cases, of which 16 were histologically confirmed. Eight cases contained cells positive for the epithelial marker Ber-EP4 and were diagnosed accordingly as carcinomas. FCM analysis of specimens obtained with a clinical question of Hodgkin lymphoma or T-cell lymphomas did not yield definitive data. The time lapse between sampling and analysis (12-84 hr) did not affect the results. This probably was due to the fact that all aspir...Continue Reading

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Citations

Mar 24, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Michael DictorEva Rambech
Jan 1, 2009·Journal of Cytology·Arvind RajwanshiGautam Upasana
Jul 1, 2011·Avicenna Journal of Medicine·Mousa A Al-Abbadi
Jul 21, 2011·Cytopathology : Official Journal of the British Society for Clinical Cytology·L Skoog, E Tani
Nov 10, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Awtar KrishanMehrdad Nadji
Mar 3, 2006·Diagnostic Cytopathology·Marcos Roberto Martins, Gilda da Cunha Santos

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